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Tags & Cell Markers Epitope Tags DDDDK Tag

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(ab2493)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Key features and details

  • HRP Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体
    参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗
  • 描述

    HRP兔多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence)
  • 宿主

    Rabbit
  • 偶联物

    HRP
  • 经测试应用

    适用于: ELISA, WBmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).

  • 常规说明

    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag

相关产品

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2493于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/5000 - 1/20000.
WB (1)
1/1000 - 1/10000.
说明
ELISA
1/5000 - 1/20000.
WB
1/1000 - 1/10000.

靶标

  • 相关性

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
  • 别名

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (3)

发表研究结果有使用 ab2493?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2493 被引用在 3 文献中.

  • Bernard PE  et al. Single Amino Acid Replacements in RocA Disrupt Protein-Protein Interactions To Alter the Molecular Pathogenesis of Group A Streptococcus. Infect Immun 88:N/A (2020). PubMed: 32817331
  • Kling E  et al. Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons. Nucleic Acids Res 46:7250-7260 (2018). PubMed: 29945249
  • Belashov IA  et al. Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription. Nucleic Acids Res 46:6401-6415 (2018). PubMed: 29961805

客户评价及客户问答

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Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (yeast cell lysate)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
yeast cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 26 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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