AB190564

重组HRP Anti-Cyclin D1抗体[EPR2241]

HRP Anti-Cyclin D1 antibody [EPR2241]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal Cyclin D1 antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

查看别名

BCL1, PRAD1, CCND1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1

3 Images

Lab

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk blocking buffer before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution

Lane 1:

MCF7 Whole Cell Lysate at 10 µg

Lane 2:

RAW 264.7 Whole Cell Lysate at 10 µg

Lane 3:

PC12 Whole cell Lysate at 10 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 4min

Lab

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 1min

Lab

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (AB190564)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab190564 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin D1 antibody [EPR2241] (ab190564) at 1/5000 dilution

All lanes:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

true

Exposure time: 1min

不同偶联物与剂型 (4)

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cyclin D1 antibody [EPR2241]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cyclin D1 antibody [EPR2241]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cyclin D1 antibody [EPR2241]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cyclin D1 antibody [EPR2241]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2241

亚型

IgG

偶联物

HRP

激发波长/发射波长

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The protein expressed by the CCND1 gene acts as a regulatory component of the cyclin D1-CDK4 (DC) complex, which phosphorylates and inhibits retinoblastoma (RB) protein family members, including RB1, regulating the cell cycle during the G(1)/S transition. Phosphorylation of RB1 allows the transcription factor E2F to dissociate from the RB/E2F complex, enabling transcription of E2F target genes responsible for G(1) phase progression. In early G(1) phase, it hypophosphorylates RB1. Cyclin D-CDK4 complexes integrate various mitogenic and antimitogenic signals. The protein also serves as a substrate for SMAD3, phosphorylating it in a cell-cycle-dependent manner to repress its transcriptional activity, and forms part of the cyclin D1/CDK4/CDKN1B complex necessary for nuclear translocation and activity. Additionally, it exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters, independent of the cell cycle. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CCND1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 13:633495 PubMed33732137

2021

Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Cong Qi,Xing-Xing Chen,Xin-Ran Gao,Jing-Xian Xu,Sen Liu,Jin-Fang Ge

International journal of molecular medicine 46:1135-1145 PubMed32705160

2020

Propofol‑induced HOXA11‑AS promotes proliferation, migration and invasion, but inhibits apoptosis in hepatocellular carcinoma cells by targeting miR‑4458.

Applications

Unspecified application

Species

Unspecified reactive species

Furong Song,Jun Liu,Yawei Feng,Yi Jin

Cell reports 30:2237-2247.e6 PubMed32075762

2020

OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lien Verboom,Arne Martens,Dario Priem,Esther Hoste,Mozes Sze,Hanna Vikkula,Lisette Van Hove,Sofie Voet,Jana Roels,Jonathan Maelfait,Laura Bongiovanni,Alain de Bruin,Charlotte L Scott,Yvan Saeys,Manolis Pasparakis,Mathieu J M Bertrand,Geert van Loo

Communications biology 2:307 PubMed31428695

2019

Targeting p16-induced senescence prevents cigarette smoke-induced emphysema by promoting IGF1/Akt1 signaling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher T Cottage,Norman Peterson,Jennifer Kearley,Aaron Berlin,Ximing Xiong,Anna Huntley,Weiguang Zhao,Charles Brown,Annik Migneault,Kamelia Zerrouki,Gerald Criner,Roland Kolbeck,Jane Connor,Raphael Lemaire

View all publications

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