HRP Anti-CD90 / Thy1抗体[MRC OX-7] (ab199219)
Key features and details
- HRP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: WB
- Reacts with: Rat
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
HRP Anti-CD90 / Thy1抗体[MRC OX-7]
参阅全部 CD90 / Thy1 一抗 -
描述
HRP小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Rabbit, Guinea pig -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Rat brain tissue lysate and PC12 whole cell lysate.
-
常规说明
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
MRC OX-7 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Alternative Versions
- PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
- PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab199219于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
Observed molecular weight may vary depending on the glycosylation level of the target. |
说明 |
---|
WB
1/5000. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa). Observed molecular weight may vary depending on the glycosylation level of the target. |
靶标
-
功能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
- Unigene: 3951 Mouse
- Unigene: 108198 Rat
-
别名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
图片
-
All lanes : HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219) at 1/5000 dilution
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199219 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab199219 尚未被引用在任何文献中。