AB195017

重组HRP Anti-CD34抗体[EP373Y]

HRP Anti-CD34 antibody [EP373Y]

BOND RX™ Validated
RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal CD34 antibody - conjugated to HRP. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

CD34, Hematopoietic progenitor cell antigen CD34

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD34 antibody [EP373Y] (AB195017)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD34 antibody [EP373Y] (AB195017)

IHC image of CD34 staining in a section of formalin-fixed paraffin-embedded normal human umbilical cord tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195017 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

不同偶联物与剂型 (9)

578 PE

PE Anti-CD34 antibody [EP373Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD34 antibody [EP373Y]
660 APC

APC Anti-CD34 antibody [EP373Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD34 antibody [EP373Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD34 antibody [EP373Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD34 antibody [EP373Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD34 antibody [EP373Y]
Unconjugated

Anti-CD34 antibody [EP373Y]
Carrier free

Anti-CD34 antibody [EP373Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP373Y

亚型

IgG

偶联物

HRP

激发波长/发射波长

不含载体蛋白

反应种属

Human, Human, Mouse, Rat

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<a href='/products/primary-antibodies/hrp-rabbit-igg-monoclonal-epr25a-isotype-control-ab199507'>ab199507</a> - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

See full target information CD34

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