HRP Anti-beta Actin抗体[mAbcam 8226] - Loading Control (ab20272)
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.cn/ps/products/20/ab20272/Images/ab20272-29577-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg "Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)")
Key features and details
- HRP Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
HRP Anti-beta Actin抗体[mAbcam 8226] - Loading Control
参阅全部 beta Actin 一抗 -
描述
HRP小鼠单克隆抗体[mAbcam 8226] to beta Actin - Loading Control -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Drosophila melanogaster, Monkey, African green monkey, Chinese hamster
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
mAbcam 8226 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184092)
- Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab184576)
- Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free (ab264083)
- Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
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Recombinant Protein
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Related Products
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- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20272于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (17) |
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.
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| 说明 |
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WB
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details. |
靶标
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功能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
疾病相关
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
序列相似性
Belongs to the actin family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 100033878 Horse
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
see all -
别名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
图片
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Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : human testis lysate
Lane 2 : human brain lysate
Lane 3 : human tonsil lysate
Lane 4 : mouse brain lysate
Lane 5 : mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Exposure time: 2 minutes -
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.cn/ps/products/20/ab20272/Images/ab20272-29576-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : 3T3 whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds -
![Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](https://www.abcam.cn/ps/products/20/ab20272/Images/ab20272-29575-anti-beta-actin-antibody-mabcam-8226-loading-control-hrp-western-blot.jpg)
Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)Lanes 1-4 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
Lanes 5-8 : HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% MILK)
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lanes 3 & 7 : NIH/3T3 whole cell lysate (ab7179)
Lanes 4 & 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
数据表及文件
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SDS download
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Datasheet download
文献 (296)
ab20272 被引用在 296 文献中.
- Zhang X et al. Impaired autophagy-accelerated senescence of alveolar type II epithelial cells drives pulmonary fibrosis induced by single-walled carbon nanotubes. J Nanobiotechnology 21:69 (2023). PubMed: 36849924
- Watanabe-Yasuoka Y et al. Lactiplantibacillus plantarum OLL2712 Induces Autophagy via MYD88 and Strengthens Tight Junction Integrity to Promote the Barrier Function in Intestinal Epithelial Cells. Nutrients 15:N/A (2023). PubMed: 37375559
- Greig JA et al. Lipid nanoparticle-encapsulated mRNA therapy corrects serum total bilirubin level in Crigler-Najjar syndrome mouse model. Mol Ther Methods Clin Dev 29:32-39 (2023). PubMed: 36936447
- Almási N et al. Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants. Antioxidants (Basel) 12:N/A (2023). PubMed: 37627526
- Balaraman V et al. Identification of Host Factors for Rift Valley Fever Phlebovirus. Viruses 15:N/A (2023). PubMed: 38005928