AB20272

HRP Anti-beta Actin抗体[mAbcam 8226] - Loading Control

Name: HRP Anti-beta Actin抗体[mAbcam 8226] - Loading Control (ab20272)| Abcam中文官网
Brand: Abcam Limited
SKU: AB20272
Rating: 5 (17 reviews)

HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

(17 Reviews)

(296 Publications)

Anti-beta Actin antibody [mAbcam 8226] - Loading Control - HRP conjugated (ab20272) is a mouse monoclonal antibody detecting beta Actin in Western Blot. Suitable for Human, Mouse, Rat.

- Over 290 publications
- Trusted since 2005

查看别名

Beta-actin, ACTB

3 Images

Unknown

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lane 1:

human testis lysate at 20 µg

Lane 2:

human brain lysate at 20 µg

Lane 3:

human tonsil lysate at 20 µg

Lane 4:

mouse brain lysate at 20 µg

Lane 5:

mouse spleen lysate at 20 µg

Predicted band size: 41 kDa

true

Exposure time: 2min

Project1272****

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

3T3 whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

Exposure time: 30s

Project6577****

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.

All lanes:

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) at 1/5000 dilution

Lanes 1 and 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lanes 2 and 6:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lanes 3 and 7:

Western blot - NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lanes 4 and 8:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

Exposure time: 1min

不同偶联物与剂型 (4)

Unconjugated

Anti-beta Actin antibody [mAbcam 8226] - Loading Control
Carrier free

Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free
805 Alexa Fluor® 790

Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control
702 Alexa Fluor® 680

Alexa Fluor® 680 Anti-beta Actin antibody [mAbcam 8226] - Loading Control

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

mAbcam 8226

亚型

IgG1

轻链类型

kappa

偶联物

HRP

激发波长/发射波长

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details." }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

What is this antibody validated in?
HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of beta Actin?
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) is often used as a loading control in Western blot. It specifically detects a band for beta Actin (UniProt: P60709) at a molecular weight of 42kDa.

Trusted by the scientific community
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) was first used in a scientific publication in 2005 and has been cited over 290 times in peer-reviewed journals.

Reviewed by scientists
Anti-beta Actin [mAbcam 8226] - Loading Control HRP (ab20272) has over 15 independent reviews from customers.

Related products
Antibody clone mAbcam 8226 is also available pre-conjugated to a variety of labels for your convenience – Anti-beta Actin HRP [mAbcam 8226] - Loading Control (ab20272).

Other related products
We have a range of other formats of antibody clone [mAbcam 8226] also available for your convenience: ab8226, HRP - ab20272, Alexa Fluor® 680 - ab184092, Alexa Fluor® 790 - ab184576, Carrier free - ab264083

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性能和储存信息

形式

Liquid

纯度

IgG fraction

存储溶液

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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文献 (296)

Recent publications for all applications. Explore the full list and refine your search

Endocrine regulations 57:279-291 PubMed38127690

2023

Nicotinamide prevention in diabetes-induced alterations in the rat liver.

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Unspecified application

Species

Unspecified reactive species

Tamara Kuchmerovska,Lesya Yanitska,Oksana Horkunenko,Mykhailo Guzyk,Tetiana Tykhonenko,Irina Pryvrotska

Nanomaterials (Basel, Switzerland) 13: PubMed38133063

2023

Intravenous Injection of PEI-Decorated Iron Oxide Nanoparticles Impacts NF-kappaB Protein Expression in Immunologically Stressed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Schwarz,Julia Göring,Cordula Grüttner,Ingrid Hilger

Viruses 15: PubMed38005928

2023

Identification of Host Factors for Rift Valley Fever Phlebovirus.

Applications

Unspecified application

Species

Unspecified reactive species

Velmurugan Balaraman,Sabarish V Indran,Yonghai Li,David A Meekins,Laxmi U M R Jakkula,Heidi Liu,Micheal P Hays,Jayme A Souza-Neto,Natasha N Gaudreault,Philip R Hardwidge,William C Wilson,Friedemann Weber,Juergen A Richt

Experimental & molecular medicine 55:2332-2345 PubMed37907736

2023

Lactobacillus plantarum ameliorates NASH-related inflammation by upregulating L-arginine production.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Yun Kim,Jun Yong Park,Heon Yung Gee

iScience 26:108143 PubMed37915599

2023

Oral vancomycin treatment suppresses gut trypsin activity and preserves intestinal barrier function during EAE.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Bianchimano,Kacper Iwanowski,Emma M Smith,Adam Cantor,Paola Leone,Gerold Bongers,Carlos G Gonzalez,Yoon Hongsup,Joshua Elias,Howard L Weiner,Jose C Clemente,Stephanie K Tankou

Journal of biochemical and molecular toxicology 37:e23510 PubMed37700718

2023

NOD2 silencing promotes cell apoptosis and inhibits drug resistance in chronic lymphocytic leukemia by inhibiting the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiping Zheng,Zongsi Zhu,Shanshan Weng,Qikai Zhang,Yixiao Fu,Xiaoping Cai,Zhen Liu,Yuejian Shi

Antioxidants (Basel, Switzerland) 12: PubMed37627526

2023

Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants.

Applications

Unspecified application

Species

Unspecified reactive species

Nikoletta Almási,Szilvia Török,Amin Al-Awar,Médea Veszelka,László Király,Denise Börzsei,Renáta Szabó,Csaba Varga

Nutrients 15: PubMed37375559

2023

OLL2712 Induces Autophagy via MYD88 and Strengthens Tight Junction Integrity to Promote the Barrier Function in Intestinal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yumiko Watanabe-Yasuoka,Ayako Gotou,Shigeomi Shimizu,Toshihiro Sashihara

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 131:313-324 PubMed37185991

2023

Circ_0006873 suppresses the osteogenic differentiation of human-derived mesenchymal stem cells through mediating miR-20a/SMURF2 axis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangna Pang,Yongfu Wu,Yanlin Ji,Yilan Si,Fang Liang

Nutrients 15: PubMed37049468

2023

Sesamin Metabolites Suppress the Induction of Cellular Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Chie Araki,Daisuke Takemoto,Yoshinori Kitagawa,Norifumi Tateishi,Tomohiro Rogi,Takayuki Izumo,Shimpei Kawamoto,Hiroshi Shibata,Eiji Hara,Masaaki Nakai

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

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alexa-fluor-680-beta-actin-antibody-mabcam-8226-loading-control-ab184092

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-beta Actin抗体[mAbcam 8226] - Loading Control

HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

Western blot - HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (AB20272)

不同偶联物与剂型 (4)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原

反应性数据

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为什么推荐这个？

产品详情

性能和储存信息

形式

纯度

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (296)

Abcam Product Promise

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