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Anti-HRP抗体(ab2116)

Submit a review Q&A (2)References (2)

Key features and details

  • Sheep polyclonal to HRP
  • Suitable for: ELISA, Conjugation, IP, WB
  • Reacts with: Horseradish
  • Isotype: IgG

概述

  • 产品名称

    Anti-HRP抗体
    参阅全部 HRP 一抗

  • 描述

    羊多克隆抗体to HRP

  • 宿主

    Sheep

  • 经测试应用

    适用于: ELISA, Conjugation, IP, WBmore details

  • 种属反应性

    与反应: Horseradish

  • 免疫原

    Full length native protein (purified) corresponding to HRP. Horseradish peroxidase

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Lyophilized:Restore with 100ul deionized water

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.2
    Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...

  • 纯度

    Ion Exchange Chromatography

  • 纯化说明

    Anti-PEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2116于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/2000 - 1/10000.
Conjugation
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
1/500 - 1/3000.
说明
ELISA
1/2000 - 1/10000.
Conjugation
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
1/500 - 1/3000.

靶标

  • 相关性

    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

  • 细胞定位

    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

  • 数据库链接

    • 别名

      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    文献 (2)

    发表研究结果有使用 ab2116？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2116 被引用在 2 文献中.

    • Evers L  et al. Identification of Dysregulated microRNAs in Glioblastoma Stem-like Cells. Brain Sci 13:N/A (2023). PubMed: 36831894
    • Li YF  et al. Targeting MRP4 expression by anti-androgen treatment reverses MRP4-mediated docetaxel resistance in castration-resistant prostate cancer. Oncol Lett 14:1748-1756 (2017). PubMed: 28789405

    客户评价及客户问答

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    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    is this antiserum suitable for inhibiting peroxidase activity?

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    Answer

    The antibody has not been tested for inhibition of peroxidase. We have introduced a new scheme to allow researchers to try an antibody in an unknown application 'risk free'. If you want to place an order with our reference LD/NS.02.08.01 we can include the antibody in this scheme (No#2 below applies in this case). 1.) Abcam will refund the product if it does not work in the application and species as stated on the datasheet. 2.) If it is used in an application or on a species that is not listed on the datasheet then Abcam will refund or replace the product for another of equal value as long as Abcam receives the negative data for publishing on the datasheets to help other researchers.

    Read More

    Question

    I have tumours grown using transfectants stably expressing the HRP enzyme and would like to stain using immunohistochemistry. Are any of your horseradish peroxidase antibodies suitable for IHC in either frozen or paraffin embeded sections, and would they also pick up other peroxidases present in the tissue such as myeloperoxidase?

    Read More
    Answer

    We do not have any reports of any of our HRP antibodies being used as a primary antibody with frozen or paraffin embedded sections. In addition, cross-reactivity with endogenous tissue peroxidases has not been tested.

    Read More

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