Anti-HRP抗体(ab181658)
Key features and details
- Goat polyclonal to HRP
- Suitable for: WB
- Reacts with: Horseradish
- Isotype: IgG
概述
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产品名称
Anti-HRP抗体
参阅全部 HRP 一抗 -
描述
山羊多克隆抗体to HRP -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Horseradish -
免疫原
Full length native protein (purified) corresponding to HRP. (Horseradish).
Database link: P00433 -
阳性对照
- Horseradish peroxidase
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
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- 450 nm Stop Solution for TMB Substrate (ab171529)
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- Immunoassay Blocking Buffer (ab171534)
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181658于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 39 kDa.
|
说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 39 kDa. |
靶标
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相关性
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
细胞定位
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
数据库链接
- SwissProt: P00433 Horseradish
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别名
- Horseradish Peroxidase antibody
- HPRC1 antibody
- HRP antibody
see all
图片
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All lanes : Anti-HRP antibody (ab181658) at 1/5000 dilution
Lane 1 : Horseradish peroxidase (reducing conditions) at 1 µg
Lane 2 : Horseradish peroxidase (reducing conditions) at 0.25 µg
Lane 3 : Horseradish peroxidase (non-reducing conditions) at 1 µg
Lane 4 : Horseradish peroxidase (non-reducing conditions) at 0.25 µg
Secondary
All lanes : Dylight 649 conjugated Donkey anti Goat at 1/10000 dilution
Predicted band size: 39 kDaReduced samples of purified Horseradish peroxidase contained 4% BME and were boiled for 5 minutes.
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Anti-HRP antibody (ab181658) at 1/1000 dilution + Peroxidase at 0.05 µg
Secondary
DyLight™ 649 goat secondary at 1/20000 dilution
Predicted band size: 39 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab181658 被引用在 25 文献中.
- Liang X et al. Dickkopf‑3 and β‑catenin play opposite roles in the Wnt/β‑catenin pathway during the abnormal subchondral bone formation of human knee osteoarthritis. Int J Mol Med 49:N/A (2022). PubMed: 35137918
- Long J et al. miR-500a-3p is a Potential Prognostic Biomarker in Hepatocellular Carcinoma. Int J Gen Med 15:1891-1899 (2022). PubMed: 35221718
- Tan Y et al. Circular RNA ZFR promotes cell cycle arrest and apoptosis of colorectal cancer cells via the miR-147a/CACUL1 axis. J Gastrointest Oncol 13:1793-1804 (2022). PubMed: 36092343
- Zhang J et al. Microrchidia family CW-type zinc finger 2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N-myc downstream regulated gene 1 promoter. Int J Mol Med 49:N/A (2022). PubMed: 34913078
- Zhou L et al. Silencing circ-BIRC6 inhibits the proliferation, invasion, migration and epithelial-mesenchymal transition of bladder cancer cells by targeting the miR-495-3p/XBP1 signaling axis. Mol Med Rep 24:N/A (2021). PubMed: 34542161