HRP Anti-alpha Tubulin抗体(ab106043)
Key features and details
- HRP Rabbit polyclonal to alpha Tubulin
- Suitable for: ELISA, WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
HRP Anti-alpha Tubulin抗体
参阅全部 alpha Tubulin 一抗 -
描述
HRP兔多克隆抗体to alpha Tubulin -
宿主
Rabbit -
偶联物
HRP -
特异性
Anti-Tubulin is directed against human alpha Tubulin protein. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
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经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Human
预测可用于: a wide range of other species
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免疫原
Synthetic peptide:
ALEKDYEEVGVDSVE
, corresponding to amino acids 427-441 of Human alpha Tubulin. -
常规说明
Do NOT add Sodium Azide!The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Restore with 0.1 mL of deionized water (or equivalent). -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is Immunoglobulin and Protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Anti-Tubulin is directed against human alpha Tubulin protein. The Tubulin Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106043于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/20000.
|
|
| WB |
1/1000 - 1/5000.
|
| 说明 |
|---|
|
ELISA
1/20000. |
|
WB
1/1000 - 1/5000. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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别名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
数据表及文件
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Datasheet download
文献 (1)
ab106043 被引用在 1 文献中.
- Yang YH et al. Stromal Tissue Rigidity Promotes Mesenchymal Stem Cell-Mediated Corneal Wound Healing Through the Transforming Growth Factor ß Signaling Pathway. Stem Cells 34:2525-2535 (2016). PubMed: 27250866
