重组HRP Anti-6X His tag®抗体[EPR20547] (ab237339)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR20547] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-6X His tag®抗体[EPR20547]
参阅全部 6X His tag® 一抗 -
描述
HRP兔单克隆抗体[EPR20547] to 6X His tag® -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20547 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)
- Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (ab237336)
- Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
- PE Anti-6X His tag® antibody [EPR20547] (ab237338)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab237339于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000 - 1/50000.
|
说明 |
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WB
1/5000 - 1/50000. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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All lanes : HRP Anti-6X His tag® antibody [EPR20547] (ab237339) at 1/50000 dilution
Lane 1 : HEK-293 transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag
Lysates/proteins at 15 µg per lane.
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
All lanes : HRP Anti-6X His tag® antibody [EPR20547] (ab237339) at 1/5000 dilution
Lane 1 : Histone H3 recombinant (ab198757) (4 minute exposure) cell lysate
Lane 2 : Histone H4 recombinant (ab198115) (1 minute 30 seconds exposure) cell lysate
Lane 3 : Histone H2A recombinant (ab200295)(1 minute 30 seconds exposure) cell lysate
Lysates/proteins at 2 µg per lane.
Performed under reducing conditions.
Observed band size: 17 kDa why is the actual band size different from the predicted?ab237339 was shown to react with 6X His tag® in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab237339 overnight at 4°C at a 1 in 5000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab237339 被引用在 1 文献中.
- Xu Y et al. A small Rho GTPase OsRacB is required for pollen germination in rice. Dev Growth Differ 64:88-97 (2022). PubMed: 34519039