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Tags & Cell Markers Epitope Tags His Tag

HRP Anti-6X His tag®抗体(ab1269)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (8)

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Key features and details

  • HRP Goat polyclonal to 6X His tag®
  • Suitable for: WB, ELISA, ICC
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    HRP Anti-6X His tag®抗体
    参阅全部 6X His tag® 一抗
  • 描述

    HRP山羊多克隆抗体to 6X His tag®
  • 宿主

    Goat
  • 偶联物

    HRP
  • 经测试应用

    适用于: WB, ELISA, ICCmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • His Tag

相关产品

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1269于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
1/1000 - 1/30000.
ELISA (1)
1/10000 - 1/100000.
ICC
1/200 - 1/500.
说明
WB
1/1000 - 1/30000.
ELISA
1/10000 - 1/100000.
ICC
1/200 - 1/500.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.
  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (8)

发表研究结果有使用 ab1269?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1269 被引用在 8 文献中.

  • Shen M  et al. Structural basis for the multi-activity factor Rad5 in replication stress tolerance. Nat Commun 12:321 (2021). PubMed: 33436623
  • Xu X  et al. Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump. Elife 9:N/A (2020). PubMed: 32459174
  • Lee CH  et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). PubMed: 28604720
  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . PubMed: 27378283
  • Seijsing J  et al. Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP. PLoS One 8:e81350 (2013). ELISA . PubMed: 24260574
  • de Vrij J  et al. A cathepsin-cleavage site between the adenovirus capsid protein IX and a tumor-targeting ligand improves targeted transduction. Gene Ther 19:899-906 (2012). PubMed: 22011643
  • Jevnikar Z  et al. Cathepsin X cleavage of the {beta}2 integrin regulates talin-binding and LFA-1 affinity in T cells. J Leukoc Biol 90:99-109 (2011). PubMed: 21454358
  • Uil TG  et al. A lentiviral vector-based adenovirus fiber-pseudotyping approach for expedited functional assessment of candidate retargeted fibers. J Gene Med 11:990-1004 (2009). ELISA . PubMed: 19757488

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1-5 of 5 Abreviews or Q&A

Western blot abreview for HRP Anti-6X His tag® antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (His-tagged recombinant protein produced in HEK293T)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
25 µg
Specification
His-tagged recombinant protein produced in HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 24 2022

Western blot abreview for Anti-6X His tag® antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 17 2010

ELISA abreview for Anti-6X His tag® antibody (HRP)

Good
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Escherichia coli Recombinant protein
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: PBS 10% FC
Type
Direct
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Michael Aigner

Verified customer

提交于 Aug 15 2005

Western blot abreview for Anti-6X His tag® antibody (HRP)

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Recombinant protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Sunil Verma

Verified customer

提交于 Aug 05 2005

Question

Customer using Goat polyclonal to 6X His tag for ELISA. Wants to coat nickle plate with this antibody. Then detect a mutant protein with his tag on (no antibodies against this protein). Will antibody still recognise bound protien in ELISA?

Read More

Abcam community

Verified customer

Asked on Jul 20 2006

Answer

I feel the best option would be for you to bind your mutant protein to the nickel plate. The ab1269 antibody can then be used to detect the his-tag on your mutant protein. It then leaves the HRP on the antibody free for detection. If you coat the plate with the ab1269 antibody, it will bind your mutant protein, but without a secondary antibody, you may have no means of detecting this.

Read More

Abcam Scientific Support

回复于 Jul 20 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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