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Anti-HPV18 E2抗体[2E7] (ab100969)

Submit a review Submit a question References (2)
Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969)
  • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (ab100969)

Key features and details

  • Mouse monoclonal [2E7] to HPV18 E2
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Human papillomavirus
  • Isotype: IgG

概述

  • 产品名称

    Anti-HPV18 E2抗体[2E7]

  • 描述

    小鼠单克隆抗体[2E7] to HPV18 E2

  • 宿主

    Mouse

  • 特异性

    Reacts in WB, and ELISA with HPV18 native E2 protein as well recombinant E2 protein expressed in bacteria and Cos 7 cells.

  • 经测试应用

    适用于: WB, ELISA, ICC/IFmore details
    不适用于: IP

  • 种属反应性

    与反应: Human papillomavirus

  • 免疫原

    Recombinant full length protein corresponding to HPV18 E2.

  • 表位

    Mapped to amino acids 1-83.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 纯化说明

    Purified from bacteria by affinity chromatography.

  • 克隆

    单克隆

  • 克隆编号

    2E7

  • 同种型

    IgG

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab100969于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA
Use a concentration of 0.1 µg/ml.
ICC/IF
Use at an assay dependent concentration.
说明
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA
Use a concentration of 0.1 µg/ml.
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for IP.

靶标

  • 相关性

    Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.

  • 细胞定位

    Host nucleus

  • 数据库链接

    • 别名

      • E2 antibody
      • Human papillomavirus type 18 E2 protein antibody
      • Regulatory protein E2 antibody

    图片

    • Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969)
      Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969)
      All lanes : Anti-HPV18 E2 antibody [2E7] (ab100969) at 1/100 dilution

      Lane 1 : lysates of U20S cells transfected with plasmid expressing HPV11 E2 protein
      Lane 2 : lysates of plain U20S cells
      Lane 3 : lysates of plain HeLa cells
      Lane 4 : lysates of U20S transfected with plasmid expressing HPV11 E2 protein
      Lane 5 : marker

      Predicted band size: 41 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (ab100969)
      Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (ab100969)
      Immunofluorescence (IF) analysis of HPV18 E2 protein in U2OS cells. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E2 detected by ab100969.

    文献 (2)

    发表研究结果有使用 ab100969？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab100969 被引用在 2 文献中.

    • Fuentes-González AM  et al. Intratype variants of the E2 protein from human papillomavirus type 18 induce different gene expression profiles associated with apoptosis and cell proliferation. Arch Virol 164:1815-1827 (2019). PubMed: 30631960
    • Cruz-Gregorio A  et al. Human Papillomavirus Types 16 and 18 Early-expressed Proteins Differentially Modulate the Cellular Redox State and DNA Damage. Int J Biol Sci 14:21-35 (2018). WB . PubMed: 29483822

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