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重组RabMAb

重组Anti-HPS1抗体[EP8429(2)] (ab174301)

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Western blot - Anti-HPS1 antibody [EP8429(2)] (ab174301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
  • Immunocytochemistry/ Immunofluorescence - Anti-HPS1 antibody [EP8429(2)] (ab174301)
  • Flow Cytometry (Intracellular) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP8429(2)] to HPS1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-HPS1抗体[EP8429(2)]
    参阅全部 HPS1 一抗

  • 描述

    兔单克隆抗体[EP8429(2)] to HPS1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human HPS1 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92902

  • 阳性对照

    • WB: SW480, A375, NIH/3T3, and C6 lysates. IHC-P: Human skeletal muscle, Human hepatocellular cancer, Mouse and Rat kidney. ICC/IF: K-562 cells. Flow Cyt (Intra): K-562 cells.

  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 9th February 2024.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP8429(2)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174301于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 79 kDa.
ICC/IF
1/50.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 79 kDa.
ICC/IF
1/50.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Component of multiple cytoplasmic organelles. Apparently crucial for their normal development and function. May be involved in intracellular protein sorting.

  • 组织特异性

    Ubiquitous.

  • 疾病相关

    Defects in HPS1 are the cause of Hermansky-Pudlak syndrome type 1 (HPS1) [MIM:203300]. Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Hermansky Pudlak syndrome 1 protein antibody
      • Hermansky Pudlak syndrome gene antibody
      • Hermansky Pudlak syndrome type 1 antibody
      • Hermansky-Pudlak syndrome 1 antibody
      • Hermansky-Pudlak syndrome 1 protein antibody
      • HPS antibody
      • HPS1 antibody
      • HPS1_HUMAN antibody
      • MGC5277 antibody
      see all

    图片

    • Western blot - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      Western blot - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      All lanes : Anti-HPS1 antibody [EP8429(2)] (ab174301) at 1/1000 dilution

      Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : A375 (Human malignant melanoma epithelial cell) whole cell lysate
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
      Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 79 kDa
      Observed band size: 79 kDa


      Exposure time: 60 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling HPS1 with ab174301 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular cancer labeling HPS1 with ab174301 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney labeling HPS1 with ab174301 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

      Immunohistochemical analysis of paraffin-embedded Rat kidney labeling HPS1 with ab174301 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      Immunocytochemistry/ Immunofluorescence - Anti-HPS1 antibody [EP8429(2)] (ab174301)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 cells labelling HPS1 with ab174301 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

    • Flow Cytometry (Intracellular) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
      Flow Cytometry (Intracellular) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 cells labelling HPS1 with ab174301 at 1/30 dilution (10 µg/mL) (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.

    文献 (0)

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