重组Anti-HPDL抗体[EPR11691] - C-terminal (ab174841)
![Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.cn/ps/products/174/ab174841/Images/ab174841-397644-anti-hpdl-antibody-epr11691-c-terminal-western-blot-human-knockout-lysate.jpg "Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11691] to HPDL - C-terminal
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HPDL抗体[EPR11691] - C-terminal
参阅全部 HPDL 一抗 -
描述
兔单克隆抗体[EPR11691] to HPDL - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC -
种属反应性
与反应: Human
预测可用于: Rat
不与反应:
Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Caco-2, MCF7 and HeLa cell lysates; Human fetal heart lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11691 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174841于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
|
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| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
|
IP
1/10 - 1/100. |
靶标
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功能
May have dioxygenase activity. -
序列相似性
Belongs to the 4HPPD family. - Information by UniProt
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数据库链接
- Entrez Gene: 84842 Human
- Entrez Gene: 313521 Rat
- SwissProt: Q96IR7 Human
- SwissProt: Q5XIH9 Rat
- Unigene: 162717 Human
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别名
- 4 HPPD L antibody
- 4-hydroxyphenylpyruvate dioxygenase-like antibody
- 4-hydroxyphenylpyruvate dioxygenase-like protein antibody
see all
图片
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Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)All lanes : Anti-HPDL antibody [EPR11691] - C-terminal (ab174841) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HPDL knockout HeLa cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaLanes 1-3: Merged signal (red and green). Green - ab174841 observed at 39 kDa. Red - loading control ab7291 observed at 50 kDa.
ab174841 Anti-HPDL antibody [EPR11691] - C-terminal was shown to specifically react with HPDL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265868 (knockout cell lysate ab257995) was used. Wild-type and HPDL knockout samples were subjected to SDS-PAGE. ab174841 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.cn/ps/products/174/ab174841/Images/ab174841-200267-anti-hpdl-antibody-epr11691-c-terminal-western-blot.jpg)
Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)All lanes : Anti-HPDL antibody [EPR11691] - C-terminal (ab174841) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
![Immunoprecipitation - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.cn/ps/products/174/ab174841/Images/ab174841-200268-anti-hpdl-antibody-epr11691-c-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, labeling HPDL, immunoprecipitated using ab174841 at 1/10 dilution.
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![Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.cn/ps/products/174/ab174841/Images/ab174841-4-benefits-of-recombinant-antibodies.png)
Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab174841 尚未被引用在任何文献中。