重组Anti-HPD抗体[EPR5297] (ab133515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5297] to HPD
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-HPD抗体[EPR5297]
参阅全部 HPD 一抗 -
描述
兔单克隆抗体[EPR5297] to HPD -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2 and Fetal liver lysates. Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.57 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5297 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133515于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250-1/50 See IHC antigen retrieval protocols. The rat recommendation is based on the WB results. This antibody may not be suitable for IHC with rat samples. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250-1/50 See IHC antigen retrieval protocols. The rat recommendation is based on the WB results. This antibody may not be suitable for IHC with rat samples. |
靶标
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功能
Key enzyme in the degradation of tyrosine. -
通路
Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6. -
疾病相关
Tyrosinemia 3
Hawkinsinuria -
序列相似性
Belongs to the 4HPPD family. - Information by UniProt
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数据库链接
- Entrez Gene: 3242 Human
- Entrez Gene: 29531 Rat
- Omim: 609695 Human
- SwissProt: P32754 Human
- SwissProt: P32755 Rat
- Unigene: 2899 Human
- Unigene: 3664 Rat
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别名
- 4 HPPD antibody
- 4 hydroxyphenylpyruvate dioxygenase antibody
- 4 hydroxyphenylpyruvic acid oxidase antibody
see all
图片
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Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HPD with purified ab133515 at 1:2000 dilution (0.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Anti-HPD antibody [EPR5297] (ab133515) at 1/5000 dilution (purified) + Rat liver tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : Fetal liver tissue lysate
Lane 3 : Human kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HPD with unpurified ab133515 at 1/250 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133515 被引用在 1 文献中.
- Johnston K et al. Isotope tracing reveals glycolysis and oxidative metabolism in childhood tumors of multiple histologies. Med 2:395-410 (2021). PubMed: 33860280