重组Anti-HP1 alpha抗体[RAB-C133] (ab317725)
![Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [RAB-C133] (ab317725)](https://www.abcam.cn/ps/products/317/ab317725/Images/ab317725-3-anti-hp1-alpha-antibody-rab-c133-immunocytochemistry-hela-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [RAB-C133] (ab317725)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [RAB-C133] to HP1 alpha
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
概述
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产品名称
Anti-HP1 alpha抗体[RAB-C133]
参阅全部 HP1 alpha 一抗 -
描述
人单克隆抗体[RAB-C133] to HP1 alpha -
宿主
Human -
特异性
This antibody recognizes CBX5 (Chromobox protein homolog 5). It binds to a folded domain, amino acids 18-75.
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经测试应用
适用于: WB, Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human HP1 alpha aa 1-100. N-terminal tag
Database link: P45973 -
阳性对照
- Flow Cyt (Intra): HeLa cells. ICC/IF: HeLa cells. WB: Nuclear lysates of HeLa, HEK293, A431, MCF7, Jurkat and K562.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified by Immobilized Metal Affinity Chromatography. -
克隆
单克隆 -
克隆编号
RAB-C133 -
同种型
unknown -
轻链类型
kappa -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317725于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
序列相似性
Contains 2 chromo domains. -
翻译后修饰
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 23468 Human
- Omim: 604478 Human
- SwissProt: P45973 Human
- Unigene: 349283 Human
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别名
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [RAB-C133] (ab317725)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of ab317725 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom primary antibody/secondary, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
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![Western blot - Anti-HP1 alpha antibody [RAB-C133] (ab317725)](https://www.abcam.cn/ps/products/317/ab317725/Images/ab317725-2-anti-hp1-alpha-antibody-rab-c133-western-blot-hela-hek293-a431-mcf7-jurkat-k562-human.jpg)
Western blot - Anti-HP1 alpha antibody [RAB-C133] (ab317725)All lanes : Chimeric rabbit version of ab317725 at 0.003 µg/ml
Lane 1 : Nuclear lysates of HeLa (35ug protein in RIPA buffer)
Lane 2 : Nuclear lysates of HEK293 (35ug protein in RIPA buffer)
Lane 3 : Nuclear lysates of A431 (35ug protein in RIPA buffer)
Lane 4 : Nuclear lysates of MCF7 (35ug protein in RIPA buffer)
Lane 5 : Nuclear lysates of Jurkat (35ug protein in RIPA buffer)
Lane 6 : Nuclear lysates of K562 (35ug protein in RIPA buffer)
Secondary
All lanes : Anti-rabbit secondary antibodyA primary incubation of 1h was used and protein was detected by chemiluminescence.
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![Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [RAB-C133] (ab317725)](https://www.abcam.cn/ps/products/317/ab317725/Images/ab317725-1-anti-hp1-alpha-antibody-rab-c133-flow-cytometry-intracellular-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [RAB-C133] (ab317725)Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of ab317725 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317725 尚未被引用在任何文献中。