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Anti-HOXD11抗体[5G4] (ab55255)

Reviews (1) Submit a question References (1)
Western blot - Anti-HOXD11 antibody [5G4] (ab55255)
  • Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)

Key features and details

  • Mouse monoclonal [5G4] to HOXD11
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-HOXD11抗体[5G4]
    参阅全部 HOXD11 一抗

  • 描述

    小鼠单克隆抗体[5G4] to HOXD11

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment: MNDFDECGQS AASMYLPGCA YYVAPSDFAS KPSFLSQPSS CQMTFPYSSN LAPHVQPVRE VAFRDYGLER AKWPYR, corresponding to amino acids 1-77 of Human HOXD11

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 27 June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.4
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 纯化说明

    Purified from TCS.

  • 克隆

    单克隆

  • 克隆编号

    5G4

  • 同种型

    IgG2a

  • 轻链类型

    kappa

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab55255于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
说明
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

  • 序列相似性

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Homeo box 4F antibody
      • Homeo box D11 antibody
      • Homeobox D11 antibody
      • Homeobox protein Hox-4F antibody
      • Homeobox protein Hox-D11 antibody
      • Hox-4F antibody
      • HOX4 antibody
      • HOX4F antibody
      • HOXD11 antibody
      • HXD11_HUMAN antibody
      see all

    图片

    • Western blot - Anti-HOXD11 antibody [5G4] (ab55255)
      Western blot - Anti-HOXD11 antibody [5G4] (ab55255)

      HOXD11 antibody (ab55255) at 1ug/lane + K-562 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)
      Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)

      ab55255 at 10 ug/ml staining HOXD11 in human cells by Immunocytochemistry/ Immunofluorescence.

      This image was generated using the ascites version of the product.

    文献 (1)

    发表研究结果有使用 ab55255？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab55255 被引用在 1 文献中.

    • Tsujimoto H  et al. A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells. Cell Rep 31:107476 (2020). PubMed: 32268094

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-HOXD11 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (embryonic limb tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    embryonic limb tissue
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3µg/mL · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Racheal Paul

    Verified customer

    提交于 Jan 26 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com