Anti-HOXC9抗体[HOXCA6E6] (ab50839)
![Western blot - Anti-HOXC9 antibody [HOXCA6E6] (ab50839)](https://www.abcam.cn/ps/products/50/ab50839/Images/ab50839-62541-anti-hoxc9-antibody-hoxca6e6-western-blot.jpg "Western blot - Anti-HOXC9 antibody [HOXCA6E6] (ab50839)")
Key features and details
- Mouse monoclonal [HOXCA6E6] to HOXC9
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-HOXC9抗体[HOXCA6E6]
参阅全部 HOXC9 一抗 -
描述
小鼠单克隆抗体[HOXCA6E6] to HOXC9 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
HOXC9 recombinant fragment (Human), containing 50-200 aa
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阳性对照
- Hela cells and HT-1080 whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
HOXCA6E6 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50839于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (4) |
1/5.
|
| WB | (1) |
1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| 说明 |
|---|
|
ICC/IF
1/5. |
|
WB
1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
靶标
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功能
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
序列相似性
Belongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3225 Human
- Omim: 142971 Human
- SwissProt: P31274 Human
- Unigene: 658823 Human
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别名
- Homeo box C9 antibody
- Homeobox C9 antibody
- Homeobox protein Hox C9 antibody
see all
图片
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Western blot - Anti-HOXC9 antibody [HOXCA6E6] (ab50839)Anti-HOXC9 antibody [HOXCA6E6] (ab50839) at 1/100 dilution + HT-1080 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-HOXC9 antibody [HOXCA6E6] (ab50839)](https://www.abcam.cn/ps/products/50/ab50839/Images/ab50839-62540-anti-hoxc9-antibody-hoxca6e6-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HOXC9 antibody [HOXCA6E6] (ab50839)ab50839 at a 1/5 dilution staining HOXC9 in Hela cells using Alexa Fluor® 488 Goat Anti-mouse IgG at a 1/200 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab50839 被引用在 11 文献中.
- Xue W et al. Generation of dorsoventral human spinal cord organoids via functionalizing composite scaffold for drug testing. iScience 26:105898 (2023). PubMed: 36647382
- Gogolou A et al. Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor. Elife 11:N/A (2022). PubMed: 36154671
- Wind M & Tsakiridis A In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells. Curr Protoc 1:e244 (2021). PubMed: 34547185
- Faustino Martins JM et al. Self-Organizing 3D Human Trunk Neuromuscular Organoids. Cell Stem Cell 26:172-186.e6 (2020). PubMed: 31956040
- Frith TJR & Tsakiridis A Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate. Curr Protoc Stem Cell Biol N/A:e81 (2019). PubMed: 30688409