Anti-HORMAD2抗体(ab272546)
Key features and details
- Rabbit polyclonal to HORMAD2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HORMAD2抗体 -
描述
兔多克隆抗体to HORMAD2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Dog
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免疫原
Recombinant fragment corresponding to Human HORMAD2 aa 1-150.
Database link: Q8N7B1 -
阳性对照
- IHC-P: Human testis tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272546于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in meiotic progression. -
组织特异性
Expressed in testis, liver and brain, and at lower levels in kidney. -
序列相似性
Contains 1 HORMA domain. -
细胞定位
Nucleus. Chromosome. Preferentially localizes to unsynapsed or desynapsed chromosomal regions during the male and female prophase I stage of meiosis. TRIP13 is required for depletion from synapsed chromosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 150280 Human
- SwissProt: Q8N7B1 Human
- Unigene: 120391 Human
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别名
- 4930529M09Rik antibody
- CT46.2 antibody
- HORM2_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD2 antibody (ab272546)
Immunohistochemical analysis of human testis tissue labelled for HORMAD-2 using ab272546 at a 1/200 dilution. Positive cytoplasmic staining onbserved in Leydig cells and weaker positive nuclear staining observed in seminiferous duct cells.
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Western blot - Anti-HORMAD2 antibody (ab272546)All lanes : Anti-HORMAD2 antibody (ab272546) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : HORMAD2 over-expression in HEK293T lysates
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272546 尚未被引用在任何文献中。