Anti-HOOK3抗体(ab173388)
Key features and details
- Mouse polyclonal to HOOK3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HOOK3抗体
参阅全部 HOOK3 一抗 -
描述
小鼠多克隆抗体to HOOK3 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Xenopus laevis
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免疫原
Recombinant full length protein corresponding to Human HOOK3 aa 1-718. (NP_115786.1).
Sequence:MFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQVLQKIDP AYFDENWLNRIKTEVGDNWRLKISNLKKILKGILDYNHEILGQQINDFTL PDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVV MTAIQELMSKESPVSAGNDAYVDLDRQLKKTTEELNEALSAKEEIAQRCH ELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQ LEQLQEETFRLEAAKDDYRIRCEELEKEISELRQQNDELTTLADEAQSLK DEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQN TVSLEEELRKANAARSQLETYKRQVVELQNRLSEESKKADKLDFEYKRLK EKVDSLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQES SDSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNEKIALLQSLLDDAN LRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEE HLEKLHEANNELQKKRAIIEDLEPRFNNSSLKIEELQEALRKKEEEMKQM EERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKE YEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQ ATSSRRSYPGHVQPATAR
Database link: Q86VS8 -
阳性对照
- HOOK3 transfected 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173388于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. |
靶标
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功能
Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. -
序列相似性
Belongs to the hook family. -
细胞定位
Cytoplasm > cytoskeleton. Golgi apparatus. Enriched at the cis-face of the Golgi complex (By similarity). Localizes to microtubule asters in prophase. - Information by UniProt
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数据库链接
- Entrez Gene: 84376 Human
- Entrez Gene: 320191 Mouse
- Entrez Gene: 443941 Xenopus laevis
- Omim: 607825 Human
- SwissProt: Q86VS8 Human
- SwissProt: Q8BUK6 Mouse
- SwissProt: Q6GQ73 Xenopus laevis
- Unigene: 162852 Human
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别名
- FLJ31058 antibody
- h-hook3 antibody
- hHK3 antibody
see all
图片
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Western blot - Anti-HOOK3 antibody (ab173388)All lanes : Anti-HOOK3 antibody (ab173388) at 1 µg/ml
Lane 1 : HOOK3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG at 1/2500 dilution
Predicted band size: 83 kDa
数据表及文件
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Datasheet download
文献 (1)
ab173388 被引用在 1 文献中.
- Villari G et al. Distinct retrograde microtubule motor sets drive early and late endosome transport. EMBO J 39:e103661 (2020). PubMed: 33215754
