重组Anti-HOOK2抗体[EPR8673] (ab133691)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8673] to HOOK2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HOOK2抗体[EPR8673]
参阅全部 HOOK2 一抗 -
描述
兔单克隆抗体[EPR8673] to HOOK2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human HOOK2 aa 1-100. The exact sequence is proprietary.
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阳性对照
- HeLa, 293T and HepG2 whole cell lysate (ab7900); Human esophageal carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.02 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8673 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133691于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
HOOK2 is a member of the Hook protein family. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 localizes to the centrosome through all phases of the cell cycle. Interference with HOOK2 function results in the loss of the radial organization of microtubules suggesting that HOOK2 contributes to the establishment and maintenance of centrosomal structure and function. HOOK2 may contribute to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes. -
细胞定位
Cytoplasmic. Note=Associates with discrete punctate structure that correspond to neither early or late endosomes, lysosomes, multivesicular bodies (MVBs), Golgi complex, endoplasmic reticulum, nor mitochondria. -
数据库链接
- Entrez Gene: 29911 Human
- Entrez Gene: 170833 Mouse
- Entrez Gene: 304669 Rat
- Omim: 607824 Human
- SwissProt: Q96ED9 Human
- SwissProt: Q7TMK6 Mouse
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别名
- A630054I03Rik antibody
- FLJ26218 antibody
- h hook2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody [EPR8673] (ab133691)
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labelling HOOK2 with ab133691 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-HOOK2 antibody [EPR8673] (ab133691) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 83 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133691 被引用在 2 文献中.
- Zhang H et al. Development and Validation of an Immune-Related Signature for the Prediction of Recurrence Risk of Patients With Laryngeal Cancer. Front Oncol 11:683915 (2021). PubMed: 34976784
- Lee H et al. Comprehensive Proteome Profiling of Platelet Identified a Protein Profile Predictive of Responses to An Antiplatelet Agent Sarpogrelate. Mol Cell Proteomics 15:3461-3472 (2016). PubMed: 27601597