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Anti-HODE抗体(ab28204)

Submit a review Q&A (3)

Key features and details

  • Goat polyclonal to HODE
  • Suitable for: RIA, ELISA
  • Reacts with: Species independent
  • Isotype: IgG

概述

  • 产品名称

    Anti-HODE抗体

  • 描述

    山羊多克隆抗体to HODE

  • 宿主

    Goat

  • 特异性

    REACTIVITY: HAPTEN REACTIVITY 9(±)-HODE 100.0% 9(S)-HODE 100.0% 9(R)-HODE 100.0% 13(S)-HODE 1.2% 13(R)-HODE 1.2% 9-oxo-ODE 1.2% 13-oxo-ODE 2.4% 11(S)-HETE 0.0% 15(S)-HETE 0.0% Linoleic Acid 0.0%

  • 经测试应用

    适用于: RIA, ELISAmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule: 9(S)-HODE; 9(R)-HODE.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab28204于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
RIA
ELISA
说明
应用说明
ELISA: Use at a concentration of 2 µg/well.
RIA: Use at a concentration of 2 µg/well.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 相关性

    The hydroxyoctadecadienoic acid (HODE) derivatives of linoleic acid, 9(R)-HODE, 9(S)-HODE and 13(S)-HODE, are the most widely distributed of the known linoleic acid metabolites. These compounds exhibit interesting biologic activities, including the regulation of platelet function, maintenance of vascular thromboresistance and transduction of the cellular responses to certain growth factors. HODE derivatives may also influence certain pathological states including, psoriasis, the development of atherosclerosis and the development of cancer. 13-S-Hydroxyoctadecadienoic acid (13-S-HODE) is the product of 15 lipoxygenase (15 LOX) metabolism of linoleic acid. It is thought to enhance cellular mitogenic response to some growth factors.

  • 细胞定位

    Secreted

  • 别名

    • 13 HODE antibody
    • 13 Hydroxyoctadecadienoic acid antibody
    • 13 S HODE antibody
    • 13 S hydroxyoctadecadienoic acid antibody
    • 13HODE antibody
    • 9 HODE antibody
    • 9 hydroxyoctadecadienoic acid antibody
    • Hydroxyoctadecadienoic Acid antibody
    see all

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab28204？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab28204 尚未被引用在任何文献中。

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Thank you very much for your information. I really appreciate your replay.

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Answer

You're very welcome. Please let me know if you need anything else! Have a great day.

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Question

I'm going to detect 9-HODE and 13-HODE in human and mouse samples with western blot. I try to find the primary anti-HODE antibody, but the product of your company  (ab28204) only shows the application on ELISA and RIA. May I use it in Western blot? Thank you,  

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Answer

Thank you for contacting us with your question. We would not recommend detecting secreted HODE in Western blot, as HODE is a very small chemical molecule and will not run properly in SDS-PAGE. ELISA will be a much more suitable assay to analyze and quantify this molecule. Ab28204 specifically reacts with 9-HODE while ab15158 reacts more specifically with S13-HODE. I hope this information will be useful, but please let me know if you have further questions and I will be happy to help.

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Question

I would like to use HODE antibody (ab28204）. I read product datasheet of ab28204 and I recognized that this antibody have reactivity against 9(±)-HODE, 9(S)-HODE,9(R)-HODE. However, there is two form of 9-HODE, 9-hydroxy-10(E),12(Z)-octadecadienoic acid (9(E,Z)-HODE) and 9-hydroxy-10(E),12(E)-octadecadienoic acid (9(E,E)-HODE). I have a question about this antibody. Which form of 9-HODE is reacted by this antibody.

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Answer

Thank you for your enquiry and I sincerely apologize for the delay in responding. I have contacted the originator of the product and they have tracked down the researcher who developed this assay. Unfortunately, I am not able to access the original paper that describe the 9-HODE from my end. However, I hope the information in the publication will provide the answers you are looking for. Here is a link to the original citations by Bull AW: http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=bull+9-hode If there is anything else that I can assist you with, please do not hesitate to contact me. Have a nice day!

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