重组Anti-hnRNP U/p120抗体[EPR12279] (ab172608)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12279] to hnRNP U/p120
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-hnRNP U/p120抗体[EPR12279]
参阅全部 hnRNP U/p120 一抗 -
描述
兔单克隆抗体[EPR12279] to hnRNP U/p120 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562, Jurkat and 293T cell lysates; Human breast carcinoma and thyroid gland carcinoma tissues; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12279 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172608于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 91 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 91 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA. -
序列相似性
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
翻译后修饰
Extensively phosphorylated.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 3192 Human
- Omim: 602869 Human
- SwissProt: Q00839 Human
- Unigene: 106212 Human
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别名
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
see all
图片
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All lanes : Anti-hnRNP U/p120 antibody [EPR12279] (ab172608) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 91 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12279] (ab172608)
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells labeling hnRNP U/p120 with ab172608 at 1/250 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab172608 被引用在 2 文献中.
- Chen X et al. Identification of HnRNP Family as Prognostic Biomarkers in Five Major Types of Gastrointestinal Cancer. Curr Gene Ther 22:449-461 (2022). PubMed: 35794744
- Miao H et al. MALAT1 modulates alternative splicing by cooperating with the splicing factors PTBP1 and PSF. Sci Adv 8:eabq7289 (2022). PubMed: 36563164