Anti-hnRNP U/p120抗体(ab20666)
Key features and details
- Rabbit polyclonal to hnRNP U/p120
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-hnRNP U/p120抗体
参阅全部 hnRNP U/p120 一抗 -
描述
兔多克隆抗体to hnRNP U/p120 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab172608.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human hnRNP U/p120 aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab21993) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 90 kDa).
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 90 kDa). |
靶标
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功能
Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA. -
序列相似性
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
翻译后修饰
Extensively phosphorylated.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 3192 Human
- Entrez Gene: 51810 Mouse
- Entrez Gene: 117280 Rat
- Omim: 602869 Human
- SwissProt: Q00839 Human
- SwissProt: Q8VEK3 Mouse
- Unigene: 106212 Human
- Unigene: 86589 Mouse
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别名
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
see all
图片
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Anti-hnRNP U/p120 antibody (ab20666) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 90 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab20666 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab20666, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Panel A shows localisation of ab20666 to the nuclei, Panel B has the Alexa Fluor® 488 channel removed for comparison.
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ab20666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab20666 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunofluorescence analysis of murine embryonic stem cells, staining hnRNP U/p120 with ab20666.
Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked in 0.1% Triton X-100/10% FCS for 20 min. Samples were incubated with primary antibody (1/500) for 2 hours before incubating with an AlexaFluor®488-conjugated goat anti-rabbit IgG (1/500) for 1 hour.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab20666 被引用在 21 文献中.
- Dugger SA et al. Neurodevelopmental deficits and cell-type-specific transcriptomic perturbations in a mouse model of HNRNPU haploinsufficiency. PLoS Genet 19:e1010952 (2023). PubMed: 37782669
- Kolpa HJ et al. SAF-A mutants disrupt chromatin structure through dominant negative effects on RNAs associated with chromatin. Mamm Genome 33:366-381 (2022). PubMed: 34859278
- Alvarez-Dominguez JR et al. An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs. iScience 25:103680 (2022). PubMed: 35036870
- Cheng D et al. The nuclear transcription factor, TAF7, is a cytoplasmic regulator of protein synthesis. Sci Adv 7:eabi5751 (2021). PubMed: 34890234
- Sharp JA et al. Cell division requires RNA eviction from condensing chromosomes. J Cell Biol 219:N/A (2020). PubMed: 33053167