Anti-hnRNP M3-M4抗体[2A6-2H3] (ab128143)
Key features and details
- Mouse monoclonal [2A6-2H3] to hnRNP M3-M4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-hnRNP M3-M4抗体[2A6-2H3]
参阅全部 hnRNP M3-M4 一抗 -
描述
小鼠单克隆抗体[2A6-2H3] to hnRNP M3-M4 -
宿主
Mouse -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Full length native Human proteins hnRNP M3-M4 (NP_005959.2, NP_112480.2)
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阳性对照
- 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, NIH 3T3 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. The product can be stored for up to 12 months. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
pH 7 to 8 -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
ab128143 was produced via Mouse Hybridoma. The hybridoma was grown in a bioreactor and the antibody was purified using a mouse immunoglobulin binding ligand. -
克隆
单克隆 -
克隆编号
2A6-2H3 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128143于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. -
序列相似性
Contains 3 RRM (RNA recognition motif) domains. -
翻译后修饰
Sumoylated. -
细胞定位
Nucleus, nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 4670 Human
- Entrez Gene: 76936 Mouse
- Omim: 160994 Human
- SwissProt: P52272 Human
- SwissProt: Q9D0E1 Mouse
- Unigene: 465808 Human
- Unigene: 666214 Human
- Unigene: 311439 Mouse
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别名
- CEA receptor antibody
- CEAR antibody
- Heterogeneous nuclear ribonucleoprotein M antibody
see all
图片
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All lanes : Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 0.04 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Lane 5 : NIH 3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30 seconds -
Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab128143 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: ab128143 at 0.04 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 77 kDa
数据表及文件
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Datasheet download
文献 (0)
ab128143 尚未被引用在任何文献中。