Anti-hnRNP K (phospho S284)抗体(ab74066)
Key features and details
- Rabbit polyclonal to hnRNP K (phospho S284)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-hnRNP K (phospho S284)抗体
参阅全部 hnRNP K 一抗 -
描述
兔多克隆抗体to hnRNP K (phospho S284) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human hnRNP K (phospho S284).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab74066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74066于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
靶标
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功能
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
序列相似性
Contains 3 KH domains. -
翻译后修饰
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 15387 Mouse
- SwissProt: P61979 Mouse
- Unigene: 142872 Mouse
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别名
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
图片
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Western blot - Anti-hnRNP K (phospho S284) antibody (ab74066)All lanes : Anti-hnRNP K (phospho S284) antibody (ab74066) at 1/500 dilution
Lane 1 : Extracts from 3T3 cells treated
with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from 3T3 cells treated
with EGF (200ng/ml, 30mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab74066 被引用在 1 文献中.
- Zhao L et al. The anticancer effects of cinobufagin on hepatocellular carcinoma Huh-7 cells are associated with activation of the p73 signaling pathway. Mol Med Rep 19:4119-4128 (2019). PubMed: 30942456