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Anti-hnRNP K抗体[F45 P9 C7] (ab23644)

Reviews (2) Submit a question References (15)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
  • Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)

Key features and details

  • Mouse monoclonal [F45 P9 C7] to hnRNP K
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

概述

  • 产品名称

    Anti-hnRNP K抗体[F45 P9 C7]
    参阅全部 hnRNP K 一抗

  • 描述

    小鼠单克隆抗体[F45 P9 C7] to hnRNP K

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Chicken

  • 免疫原

    Synthetic peptide corresponding to Human hnRNP K aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32584)

  • 阳性对照

    • This antibody gave a positive signal in the following lysates: NIH 3T3 whole cell, MEF1 whole cell; PC12 whole cell; mouse testis tissue; mouse lung tissue. FFPE: Human colon tissue sections ICC/IF: HeLa cells

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 克隆

    单克隆

  • 克隆编号

    F45 P9 C7

  • 骨髓瘤

    Ag8.653

  • 同种型

    IgG1

  • 轻链类型

    kappa

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab23644于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 51 kDa).
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 51 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.

  • 序列相似性

    Contains 3 KH domains.

  • 翻译后修饰

    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.

  • 细胞定位

    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)

    IHC image of hnRNP K staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab23644, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Lane 1 : Marker
    Lanes 2-5 : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 1 µg/ml

    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µg
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg
    Lane 4 : Jurkat whole cell lysate (ab7899) at 20 µg
    Lane 5 : HEK-293 whole cell lysate (ab7902) at 20 µg

    Secondary
    Lanes 2-5 : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    ab23644 staining hnRNP K in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab23644 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Formalin-fixed, paraffin embedded tissue sections from human normal colon or human colon carcinoma stained with ab23644 mouse monoclonal to hnRNP K. hnRNP K expression is increased in the tumour tissue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)This image is courtesy of an Abreview submitted by Satyendra Tripathi
    ab23644 staining hnRNP K in Human head and neck tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation with Tris EDTA (pH9). Samples were incubated with primary antibody (1 µg/ml in TBST) for 16 hours at 4°C. An undiluted HRP-conjugated Rabbit anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Western blot - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    All lanes : Anti-hnRNP K antibody [F45 P9 C7] (ab23644) at 5 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate
    Lane 4 : Lung (Mouse) Tissue Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?
    Additional bands at: 21 kDa, 24 kDa, 54 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [F45 P9 C7] (ab23644)
    Overlay histogram showing HeLa cells stained with ab23644 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23644, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献 (15)

发表研究结果有使用 ab23644？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab23644 被引用在 15 文献中.

  • Ou M  et al. HOXA cluster antisense RNA 2 elevates KIAA1522 expression through microRNA-520d-3p and insulin like growth factor 2 mRNA binding protein 3 to promote the growth of vascular smooth muscle cells in thoracic aortic aneurysm. ESC Heart Fail 9:2955-2966 (2022). PubMed: 35730141
  • Bampton A  et al. HnRNP K mislocalisation is a novel protein pathology of frontotemporal lobar degeneration and ageing and leads to cryptic splicing. Acta Neuropathol 142:609-627 (2021). PubMed: 34274995
  • Priyanka P  et al. The lncRNA HMS recruits RNA-binding protein HuR to stabilize the 3'-UTR of HOXC10 mRNA. J Biol Chem 297:100997 (2021). PubMed: 34302808
  • Suzuki T  et al. A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing. Nucleic Acids Res 49:e40 (2021). PubMed: 33503245
  • Pellegrini L  et al. Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice. Hum Mol Genet 27:3257-3271 (2018). PubMed: 29917075
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-hnRNP K antibody [F45 P9 C7]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain Frontal Cortex)
Specification
Brain Frontal Cortex
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 21 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-hnRNP K antibody [F45 P9 C7]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Head and neck)
Specification
Head and neck
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Satyendra Tripathi

Verified customer

提交于 Sep 14 2010

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