Name: Anti-hnRNP K antibody [EP943Y]
Brand: RabMAb
SKU: ab52600
Rating: 4 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP943Y] to hnRNP K
Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

产品名称

Anti-hnRNP K抗体[EP943Y]
参阅全部 hnRNP K 一抗
描述

兔单克隆抗体[EP943Y] to hnRNP K
宿主

Rabbit
特异性

This antibody detects both phosphorylated and unphosphorylated hnRNP K.
经测试应用

适用于: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide within Human hnRNP K (N terminal). The exact sequence is proprietary.
阳性对照
- WB:Jurkat cell lysate; ICC/IF: HeLa and MCF7 cells; Flow Cyt (intra): HeLa cells; IHC-P: Human breast carcinoma, Human lung carcinoma, Mouse stomach, and Rat stomach tissues; IP: Jurkat
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

形式

Liquid
存放说明

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP943Y
同种型

IgG
研究领域

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
Recombinant Protein
- Recombinant Human hnRNP K protein (ab132460)

The Abpromise guarantee

Abpromise™承诺保证使用ab52600于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(1)	1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
IP		1/20. For unpurified use at 1/50
IHC-P	(1)	1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF		1/50. For unpurified use at 1/100 - 1/250.
Flow Cyt (Intra)		1/20.

说明
WB 1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
IP 1/20. For unpurified use at 1/50
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF 1/50. For unpurified use at 1/100 - 1/250.
Flow Cyt (Intra) 1/20.

功能

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
序列相似性

Contains 3 KH domains.
翻译后修饰

Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
细胞定位

Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
Target information above from: UniProt accession P61978 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3190 Human
- Entrez Gene: 15387 Mouse
- Entrez Gene: 117282 Rat
- Omim: 600712 Human
- SwissProt: P61978 Human
- SwissProt: P61979 Mouse
- SwissProt: P61980 Rat
- Unigene: 522257 Human
- Unigene: 142872 Mouse
- Unigene: 11854 Rat
see all
别名
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
- Heterogeneous nuclear ribonucleoprotein K antibody
- hnRNP K antibody
- HNRNPK antibody
- HNRPK antibody
- HNRPK_HUMAN antibody
- Transformation up regulated nuclear protein antibody
- Transformation up-regulated nuclear protein antibody
- Transformation upregulated nuclear protein antibody
- TUNP antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600)

All lanes : Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/10000 dilution (Purified)

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 4 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)

ab52600 (purified) at 1:20 dilution (2µg) immunoprecipitating hnRNP K in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52600 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52600 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1:50 dilution (1.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] (ab52600)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600)

Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution + Jurkat cell lysate at 10 µg

Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa
Observed band size: ~60 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)

Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)

Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.
Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)

ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.
For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Anti-hnRNP K antibody [EP943Y] (ab52600)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab52600？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52600 被引用在 28 文献中.

Tan Y et al. A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis. Cell Death Dis 13:401 (2022). PubMed: 35461306
Halder LD et al. Candida albicans Induces Cross-Kingdom miRNA Trafficking in Human Monocytes To Promote Fungal Growth. mBio 13:e0356321 (2021). PubMed: 35132877
Ji L et al. LINC01413/hnRNP-K/ZEB1 Axis Accelerates Cell Proliferation and EMT in Colorectal Cancer via Inducing YAP1/TAZ1 Translocation. Mol Ther Nucleic Acids 19:546-561 (2020). PubMed: 31927328
Hou L et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
Liu H et al. Involvement of the Wnt/ß-Catenin signaling pathway in the heterogenous nuclear ribonucleoprotein K-driven inhibition of proliferation and migration in head and neck squamous cell carcinoma. Oncol Lett 20:394 (2020). PubMed: 33193854

View all Publications for this product

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Application

Western blot

Sample

Human Cell lysate - whole cell (liver)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-tris)

Loading amount

20 µg

Specification

liver

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Sep 30 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Normal colon)

Specification

Normal colon

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6

Permeabilization

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 31 2011

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (28)

客户评价及客户问答

Western blot abreview for Anti-hnRNP K antibody [EP943Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-hnRNP K antibody [EP943Y]