Anti-hnRNP H抗体(ab10374)
Key features and details
- Rabbit polyclonal to hnRNP H
- Suitable for: WB, IHC-P, IP, ICC/IF, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-hnRNP H抗体
参阅全部 hnRNP H 一抗 -
描述
兔多克隆抗体to hnRNP H -
宿主
Rabbit -
特异性
The antibody was designed against hnRNPH1 and does not recognize hnRNPH3. The epitope recognized by ab10374 maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187). This product may cross-react with hnRNPH2.
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经测试应用
适用于: WB, IHC-P, IP, ICC/IF, IHC-Frmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human hnRNP H aa 400 to the C-terminus (C terminal).
Database link: 3187 -
阳性对照
- IP: HeLa whole cell lysate WB: HeLa, HEK-293T, and NIH/3T3 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10374于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP | (1) |
Use at 4-10 µg/mg of lysate.
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| ICC/IF | (2) |
Use at an assay dependent concentration. PubMed: 20020773
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 24462217
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| 说明 |
|---|
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WB
1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 4-10 µg/mg of lysate. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 20020773 |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 24462217 |
靶标
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功能
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG). -
组织特异性
Expressed ubiquitously. -
序列相似性
Contains 3 RRM (RNA recognition motif) domains. -
结构域
Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA). -
细胞定位
Nucleus > nucleoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3187 Human
- Entrez Gene: 59013 Mouse
- Entrez Gene: 140931 Rat
- Omim: 601035 Human
- SwissProt: P31943 Human
- SwissProt: O35737 Mouse
- SwissProt: Q8VHV7 Rat
- Unigene: 604001 Human
see all -
别名
- DKFZp686A15170 antibody
- Heterogeneous nuclear ribonucleoprotein H antibody
- Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
see all
图片
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Western blot - Anti-hnRNP H antibody (ab10374)All lanes : Anti-hnRNP H antibody (ab10374) at 0.2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab10374)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-hnRNP H antibody (ab10374)Detection of human hnRNP H by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells.
Lane 1: Rabbit polyclonal to hnRNP H (ab10374) at 0.2 µg/mg lysate current batch
Lane 2: Rabbit polyclonal to hnRNP H (ab10374) at 0.2 µg/mg lysate former batch.
Lane 3: Control IgG
ab10374 was used at 1 ug/mL for Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab10374 被引用在 31 文献中.
- Chen ZS et al. Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD. Nat Commun 14:8420 (2023). PubMed: 38110419
- Nasrullah U et al. The E3 Ligase TRIM25 Impairs Apoptotic Cell Death in Colon Carcinoma Cells via Destabilization of Caspase-7 mRNA: A Possible Role of hnRNPH1. Cells 12:N/A (2023). PubMed: 36611995
- You A et al. TTC22 promotes m6A-mediated WTAP expression and colon cancer metastasis in an RPL4 binding-dependent pattern. Oncogene N/A:N/A (2022). WB ; Human . PubMed: 35798874
- Kirchhof L et al. The G3BP1-UPF1-Associated Long Non-Coding RNA CALA Regulates RNA Turnover in the Cytoplasm. Noncoding RNA 8:N/A (2022). PubMed: 35893232
- Wen C et al. SRSF3 and HNRNPH1 Regulate Radiation-Induced Alternative Splicing of Protein Arginine Methyltransferase 5 in Hepatocellular Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 36499164