Anti-hnRNP H 抗体

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (AB10374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection : DAB.

• IP

Supplier Data

Immunoprecipitation - Anti-hnRNP H antibody (AB10374)

Detection of human hnRNP H by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells.

Lane 1 : Rabbit polyclonal to hnRNP H (ab10374) at 0.2 μg/mg lysate current batch

Lane 2 : Rabbit polyclonal to hnRNP H (ab10374) at 0.2 μg/mg lysate former batch.

Lane 3 : Control IgG

ab10374 was used at 1 ug/mL for Western blot.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-hnRNP H antibody (ab10374)

Predicted band size: 49 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-hnRNP H antibody (AB10374)

All lanes:

Western blot - Anti-hnRNP H antibody (ab10374) at 0.2 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 30s

• WB

CiteAb

Western blot - Anti-hnRNP H antibody (AB10374)

Western Blotting using Anti-hnRNP H antibody, ab10374. Publication image from Molkentin, J. D. et al., 2017, Nat Commun, 29192139. Legend direct from paper.

Annotating the BEX1 protein interactome. a Silver-stained polyacrylamide gel following GST-only or GST-BEX1 pull-down from cardiac protein extracts. Arrows indicate the binding proteins identified by mass-spectrometry. b Silver-stained polyacrylamide gel following control or Flag-BEX1 immunoprecipitation from neonatal rat cardiomyocytes protein extracts. Arrows indicate the binding proteins identified by mass-spectrometry. c Schematic of the employed yeast two-hybrid screening strategy. The arrow shows the list of binding proteins identified. d Western blot following GST-BEX1 pull-down of the indicated proteins from cardiac extracts of WT hearts with or without addition of micrococcal nuclease to degrade RNA. e Western blot for the indicated 5 proteins following immunopreciptation of BEX1 protein from BEX1 transgenic hearts. Input is shown on the left and anti-BEX1 or IgG was used for the immunoprecipitations. f Western blot for BEX1 protein from a BEX1 transgenic heart after immunoprecipitation of the 5 shown endogenous proteins. The input is shown on the bottom and the IgG control is shown in the right lanes. g Immunofluorescence for BEX1 (green) in neonatal rat cardiomyocytes in unstimulated (serum-free media) or hypertrophic conditions (media containing 2% serum). Original magnification is 100x. Scale bar is 20 µm. Abbreviations : DDX3x = DEAD box helicase 3 X-linked; DDX1 = DEAD box helicase 1; hnRNPH1 = heterogeneous nuclear ribonucleoprotein H1; PHF11 = PHD-containing factor 11; HSP70 = heat shock protein 70; TFEB = transcription factor EB; NACA = Nascent Polypeptide-Associated Complex Alpha Subunit; EPRS = Glutamyl-Prolyl-tRNA Synthetase; DNAJB11 = DnaJ Heat Shock Protein Family Member B11; PAFAH1B1 = Platelet-Activating Factor Acetylhydrolase 1b Regulatory Subunit 1; KIF26b = Kinesin Family Member 26b; MAP4 = Microtubule-Associated Protein 4. All the gels show molecular weight markers in kiloDaltons

false

• WB

CiteAb

Western blot - Anti-hnRNP H antibody (AB10374)

Western Blotting using Anti-hnRNP H antibody, ab10374. Publication image from Molkentin, J. D. et al., 2017, Nat Commun, 29192139. Legend direct from paper.

Annotating the BEX1 protein interactome. a Silver-stained polyacrylamide gel following GST-only or GST-BEX1 pull-down from cardiac protein extracts. Arrows indicate the binding proteins identified by mass-spectrometry. b Silver-stained polyacrylamide gel following control or Flag-BEX1 immunoprecipitation from neonatal rat cardiomyocytes protein extracts. Arrows indicate the binding proteins identified by mass-spectrometry. c Schematic of the employed yeast two-hybrid screening strategy. The arrow shows the list of binding proteins identified. d Western blot following GST-BEX1 pull-down of the indicated proteins from cardiac extracts of WT hearts with or without addition of micrococcal nuclease to degrade RNA. e Western blot for the indicated 5 proteins following immunopreciptation of BEX1 protein from BEX1 transgenic hearts. Input is shown on the left and anti-BEX1 or IgG was used for the immunoprecipitations. f Western blot for BEX1 protein from a BEX1 transgenic heart after immunoprecipitation of the 5 shown endogenous proteins. The input is shown on the bottom and the IgG control is shown in the right lanes. g Immunofluorescence for BEX1 (green) in neonatal rat cardiomyocytes in unstimulated (serum-free media) or hypertrophic conditions (media containing 2% serum). Original magnification is 100x. Scale bar is 20 µm. Abbreviations : DDX3x = DEAD box helicase 3 X-linked; DDX1 = DEAD box helicase 1; hnRNPH1 = heterogeneous nuclear ribonucleoprotein H1; PHF11 = PHD-containing factor 11; HSP70 = heat shock protein 70; TFEB = transcription factor EB; NACA = Nascent Polypeptide-Associated Complex Alpha Subunit; EPRS = Glutamyl-Prolyl-tRNA Synthetase; DNAJB11 = DnaJ Heat Shock Protein Family Member B11; PAFAH1B1 = Platelet-Activating Factor Acetylhydrolase 1b Regulatory Subunit 1; KIF26b = Kinesin Family Member 26b; MAP4 = Microtubule-Associated Protein 4. All the gels show molecular weight markers in kiloDaltons

false

• WB

CiteAb

Western blot - Anti-hnRNP H antibody (AB10374)

Western Blotting using Anti-hnRNP H antibody, ab10374. Publication image from Molkentin, J. D. et al., 2017, Nat Commun, 29192139. Legend direct from paper.

Annotating the BEX1 protein interactome. a Silver-stained polyacrylamide gel following GST-only or GST-BEX1 pull-down from cardiac protein extracts. Arrows indicate the binding proteins identified by mass-spectrometry. b Silver-stained polyacrylamide gel following control or Flag-BEX1 immunoprecipitation from neonatal rat cardiomyocytes protein extracts. Arrows indicate the binding proteins identified by mass-spectrometry. c Schematic of the employed yeast two-hybrid screening strategy. The arrow shows the list of binding proteins identified. d Western blot following GST-BEX1 pull-down of the indicated proteins from cardiac extracts of WT hearts with or without addition of micrococcal nuclease to degrade RNA. e Western blot for the indicated 5 proteins following immunopreciptation of BEX1 protein from BEX1 transgenic hearts. Input is shown on the left and anti-BEX1 or IgG was used for the immunoprecipitations. f Western blot for BEX1 protein from a BEX1 transgenic heart after immunoprecipitation of the 5 shown endogenous proteins. The input is shown on the bottom and the IgG control is shown in the right lanes. g Immunofluorescence for BEX1 (green) in neonatal rat cardiomyocytes in unstimulated (serum-free media) or hypertrophic conditions (media containing 2% serum). Original magnification is 100x. Scale bar is 20 µm. Abbreviations : DDX3x = DEAD box helicase 3 X-linked; DDX1 = DEAD box helicase 1; hnRNPH1 = heterogeneous nuclear ribonucleoprotein H1; PHF11 = PHD-containing factor 11; HSP70 = heat shock protein 70; TFEB = transcription factor EB; NACA = Nascent Polypeptide-Associated Complex Alpha Subunit; EPRS = Glutamyl-Prolyl-tRNA Synthetase; DNAJB11 = DnaJ Heat Shock Protein Family Member B11; PAFAH1B1 = Platelet-Activating Factor Acetylhydrolase 1b Regulatory Subunit 1; KIF26b = Kinesin Family Member 26b; MAP4 = Microtubule-Associated Protein 4. All the gels show molecular weight markers in kiloDaltons

false