Anti-hnRNP D/AUF1 (phospho S83)抗体(ab61096)
Key features and details
- Rabbit polyclonal to hnRNP D/AUF1 (phospho S83)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-hnRNP D/AUF1 (phospho S83)抗体
参阅全部 hnRNP D/AUF1 一抗 -
描述
兔多克隆抗体to hnRNP D/AUF1 (phospho S83) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
-
免疫原
Synthetic peptide corresponding to Human hnRNP D/AUF1 aa 1-100 (phospho S83).
Database link: Q14103 -
阳性对照
- Human lung carcinoma
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity-purified from rabbit antiserum by affinity-chromatography, using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61096于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
IHC-P
1/50 - 1/100. |
靶标
-
功能
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. -
序列相似性
Contains 2 RRM (RNA recognition motif) domains. -
翻译后修饰
Arg-345 is dimethylated, probably to asymmetric dimethylarginine.
Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation. -
细胞定位
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. - Information by UniProt
-
数据库链接
- Entrez Gene: 3184 Human
- Entrez Gene: 11991 Mouse
- GenBank: NP_112738.1 Human
- Omim: 601324 Human
- SwissProt: Q14103 Human
- SwissProt: Q60668 Mouse
- Unigene: 480073 Human
- Unigene: 150231 Mouse
-
别名
- ARE binding protein AUFI type A antibody
- AU-rich element RNA-binding protein 1 antibody
- AUF antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP D/AUF1 (phospho S83) antibody (ab61096)1/50-1/100 ab61096 staining phosphorilated hnRNP D/AUF1 on human lung carcinoma tissue sections, in the absence or presence of the immunizing phosphopeptide.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab61096 尚未被引用在任何文献中。