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Anti-hnRNP D/AUF1抗体(ab61193)

Submit a review Q&A (3)References (27)
Western blot - Anti-hnRNP D/AUF1 antibody (ab61193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP D/AUF1 antibody (ab61193)
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab61193)

Key features and details

  • Rabbit polyclonal to hnRNP D/AUF1
  • Suitable for: ELISA, IP, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-hnRNP D/AUF1抗体
    参阅全部 hnRNP D/AUF1 一抗

  • 描述

    兔多克隆抗体to hnRNP D/AUF1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, IP, WB, ICC/IF, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic non-phosphopeptide (Human) from around the phosphorylation site of serine 83 (NSSPPR)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab61193于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration. PubMed: 20435889
WB
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF
1/500 - 1/1000.
IHC-P
1/50 - 1/100.
说明
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration. PubMed: 20435889
WB
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF
1/500 - 1/1000.
IHC-P
1/50 - 1/100.

靶标

  • 功能

    Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

  • 序列相似性

    Contains 2 RRM (RNA recognition motif) domains.

  • 翻译后修饰

    Arg-345 is dimethylated, probably to asymmetric dimethylarginine.
    Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation.

  • 细胞定位

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ARE binding protein AUFI type A antibody
    • AU-rich element RNA-binding protein 1 antibody
    • AUF antibody
    • AUF1 antibody
    • AUF1A antibody
    • Heterogeneous nuclear ribonucleoprotein D0 antibody
    • hnRNP D antibody
    • hnRNP D0 antibody
    • Hnrnpd antibody
    • hnRNPD0 antibody
    • HNRPD antibody
    • HNRPD_HUMAN antibody
    • P37 antibody
    see all

图片

  • Western blot - Anti-hnRNP D/AUF1 antibody (ab61193)
    Western blot - Anti-hnRNP D/AUF1 antibody (ab61193)
    All lanes : Anti-hnRNP D/AUF1 antibody (ab61193) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunizing peptide

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP D/AUF1 antibody (ab61193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP D/AUF1 antibody (ab61193)
    Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab61193 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab61193)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP D/AUF1 antibody (ab61193)
    ICC/IF image of ab61193 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61193, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献 (27)

发表研究结果有使用 ab61193？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab61193 被引用在 27 文献中.

  • Ma W & Hu J The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression. Mol Med 29:162 (2023). PubMed: 38041015
  • Oe S  et al. Cpeb1 expression is post-transcriptionally regulated by AUF1, CPEB1, and microRNAs. FEBS Open Bio 12:82-94 (2022). PubMed: 34480525
  • Puvvula PK  et al. Inhibiting an RBM39/MLL1 epigenomic regulatory complex with dominant-negative peptides disrupts cancer cell transcription and proliferation. Cell Rep 35:109156 (2021). PubMed: 34077726
  • Puvvula PK & Moon AM Novel Cell-Penetrating Peptides Derived From Scaffold-Attachment- Factor A Inhibits Cancer Cell Proliferation and Survival. Front Oncol 11:621825 (2021). PubMed: 33859938
  • Suzuki T  et al. Regulation of Fetal Genes by Transitions among RNA-Binding Proteins during Liver Development. Int J Mol Sci 21:N/A (2020). PubMed: 33297405
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Actually I would rather get a vial of new antibody pertain to
hnRNP D/AUF1 (ab50692).
As you know, we used (ab61193) last time.
Also It may be get an advantage us to use new one ( ab50692).

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab50692.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

First, I want to thank you for concern about this case...
Also for your suggestions...
We used Anti-hnRNP D/AUF1 antibody -( ab61193 ) which is pertain to Lot
number (GR45177-1 ) last time.
This time, I want to get a free of charge replacement of
Anti-hnRNP D/AUF1 antibody -( ab61193 ) though which is pertain to a
different Lot number please if it possible...

Read More
Answer

Thank you for your reply.

I'd be happy to send you another vial of ab61193, but unfortunately, we only have the same lot in stock (GR45177-1). I am sorry about that.

Another option would be a replacement with another hnRNP D/AUF1 such as ab50692. Please let me know if that would be a good option for you.
https://www.abcam.com/hnRNP-D-AUF1-antibody-ab50692.html

Alternatively, a credit or refund can be arranged.

I look forward to hear back from you.

Read More

Question

We ordered an AUF1 antibody from your company last week.
( Anti-hnRNP D/AUF1 antibody ) ( ab61193 )
We used it previously for immunohistochemistry ( IHC )on human
endometrium
sections and its results were pretty good.
Afterwards, we worked with it a trial Western Blot and we detected six
bands between 25 kDa and 75 kDa. Also you noticed just one single band
on 38 kDa in datasheet !
( We run it on proteins which derived from human endometrial stromal
cell culture. We diluted the ab. 1/1000 in milk 10%) ( C=Control ,
E2=Estrogen treatment P4=Progesteron treatment )
We used Peroxidase labeled Anti-Rabbit IgG from Vector Laboratories
as secondary antibody and diluted the sec. ab. 1/5000 in milk 10%.
So I send you WB results ( Results of AUF1 and Actin ) and I look
forward your solution about this situation.

Read More
Answer

Thank you for your email and for contacting us. I am sorry to hear this antibody is not providing satisfactory results in WB.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory WB results. I am not certain why so many non-specific bands are being obtained. It might bethat you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation. Please let me know which option would be best for you.

I look forward to hear back from you and resolve this case for you.

Read More

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