Anti-hnRNP A2B1抗体[DP3B3] (ab6102)
Key features and details
- Mouse monoclonal [DP3B3] to hnRNP A2B1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
参阅全部 hnRNP A2B1 一抗
描述小鼠单克隆抗体[DP3B3] to hnRNP A2B1
经测试应用适用于: IHC-Pmore details
Bacterially expressed His-hnRNPA2 fusion protein (Human).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.1% Sodium azide
Concentration information loading...
纯度Protein A purified
纯化说明Purified from supernatant.
The Abpromise guarantee
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml.
功能Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
序列相似性Contains 2 RRM (RNA recognition motif) domains.
细胞定位Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
- Information by UniProt
- Entrez Gene: 3181 Human
- Omim: 600124 Human
- SwissProt: P22626 Human
- Unigene: 487774 Human
- Heterogeneous nuclear ribonucleoprotein A2 antibody
- Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
- Heterogeneous nuclear ribonucleoprotein B1 antibody
ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab6102 被引用在 49 文献中.
- Shen Y et al. lncRNA ST3GAL6-AS1 promotes invasion by inhibiting hnRNPA2B1-mediated ST3GAL6 expression in multiple myeloma. Int J Oncol 58:N/A (2021). PubMed: 33649796
- Fu Y et al. Vitamin D receptor upregulates lncRNA TOPORS-AS1 which inhibits the Wnt/ß-catenin pathway and associates with favorable prognosis of ovarian cancer. Sci Rep 11:7484 (2021). PubMed: 33820921
- Balas MM et al. Establishing RNA-RNA interactions remodels lncRNA structure and promotes PRC2 activity. Sci Adv 7:N/A (2021). PubMed: 33853770
- Choksi A et al. Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1. Cancer Metab 9:16 (2021). PubMed: 33863392
- Pérez-Boza J et al. hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells. Cell Mol Life Sci 77:4413-4428 (2020). PubMed: 31894362