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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing

Anti-hnRNP A2B1抗体[DP3B3] (ab6102)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (49)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)

Key features and details

  • Mouse monoclonal [DP3B3] to hnRNP A2B1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-hnRNP A2B1抗体[DP3B3]
    参阅全部 hnRNP A2B1 一抗
  • 描述

    小鼠单克隆抗体[DP3B3] to hnRNP A2B1
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Bacterially expressed His-hnRNPA2 fusion protein (Human).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from supernatant.
  • 克隆

    单克隆
  • 克隆编号

    DP3B3
  • 骨髓瘤

    Sp2/0
  • 同种型

    IgG2a
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human hnRNP A2B1 protein (ab224866)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6102于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
Use a concentration of 1 µg/ml.
说明
IHC-P
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • 序列相似性

    Contains 2 RRM (RNA recognition motif) domains.
  • 细胞定位

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Target information above from: UniProt accession P22626 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3181 Human
    • Omim: 600124 Human
    • SwissProt: P22626 Human
    • Unigene: 487774 Human
    • 别名

      • Heterogeneous nuclear ribonucleoprotein A2 antibody
      • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
      • Heterogeneous nuclear ribonucleoprotein B1 antibody
      • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
      • hnRNP A2 / hnRNP B1 antibody
      • hnRNP A2 antibody
      • hnRNP A2/B1 antibody
      • hnRNP B1 antibody
      • hnRNP-A2 antibody
      • hnRNP-B1 antibody
      • hnRNPA2 antibody
      • Hnrnpa2b1 antibody
      • hnRNPB1 antibody
      • HNRPA2 antibody
      • HNRPA2B1 antibody
      • HNRPB1 antibody
      • Nuclear ribonucleoprotein particle A2 protein antibody
      • RNP A2 antibody
      • RNP B1 antibody
      • RNP-A2 antibody
      • RNP-B1 antibody
      • RNPA2 antibody
      • RNPB1 antibody
      • ROA2_HUMAN antibody
      • SNRPB1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)
      ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (49)

    发表研究结果有使用 ab6102?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6102 被引用在 49 文献中.

    • Shen Y  et al. lncRNA ST3GAL6-AS1 promotes invasion by inhibiting hnRNPA2B1-mediated ST3GAL6 expression in multiple myeloma. Int J Oncol 58:N/A (2021). PubMed: 33649796
    • Fu Y  et al. Vitamin D receptor upregulates lncRNA TOPORS-AS1 which inhibits the Wnt/ß-catenin pathway and associates with favorable prognosis of ovarian cancer. Sci Rep 11:7484 (2021). PubMed: 33820921
    • Balas MM  et al. Establishing RNA-RNA interactions remodels lncRNA structure and promotes PRC2 activity. Sci Adv 7:N/A (2021). PubMed: 33853770
    • Choksi A  et al. Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1. Cancer Metab 9:16 (2021). PubMed: 33863392
    • Pérez-Boza J  et al. hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells. Cell Mol Life Sci 77:4413-4428 (2020). PubMed: 31894362
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Retina)
    Permeabilization
    Yes - freeze/thaw
    Specification
    Retina
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 27 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Bladder Cancer)
    Specification
    Human Bladder Cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 11 2011

    Western blot abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NSC34)
    Loading amount
    10 µg
    Specification
    NSC34
    Gel Running Conditions
    Reduced Denaturing (4-12% nupage gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 12 2010

    Western blot abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    30 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 26 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-hnRNP A2B1 antibody [DP3B3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Specification
    A549
    Fixative
    Formaldehyde
    Permeabilization
    Yes - tx 100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 05 2009

    Question

    LOT NUMBER GR21753-2 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band WB from IP samples show no band for 37kDa zone also, the input lane shows two bands (strong one at 50kDa and a weak band at 37kDa) SAMPLE Rat primary hippocampal cell culture PRIMARY ANTIBODY 1:1000 diluted in 0,5% milk TBS-T Washed 3x 10min with TBS-T DETECTION METHOD ECF, 5min max ANTIBODY STORAGE CONDITIONS 4ºC (short-term) -20ºC (aliquots) SAMPLE PREPARATION RIPA supplemented with proteases inhibitors (CLAP and PMSF), phoshpatase inhibitor (NaF), DTT and RNase inhibitor Samples from IP were dissolved with sample buffer 2x and heated at 95ºC for 5min AMOUNT OF PROTEIN LOADED 50ug for Input 1000ug for IP ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE, tris-bicine, 12,5% polyacrylamide TRANSFER AND BLOCKING CONDITIONS CAPS-methanol, 40V o.n. 4ºC blocked with 5% milk TBS-Tween SECONDARY ANTIBODY Anti-Mouse 1:20000 diluted in 0,5% milk TBS-T Washed 3x 10min with TBS-T HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Adding the flow-through samples to the WB

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2011

    Answer

    Thank you for your enquiry regarding ab6102 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Band at 37 kDa in input sample indicate that the antibody is working fine and further optimizations in antibody dilutions could help getting clean band at right size. Based on the information provided I presume that antibody is unable to IP the protein. So in order to understand the problem I would like to ask few questions; - What type of beads were used for IP? e.g. protein A or G - How the IP was performed? Have you mixed antibody beads and lysates once, or beads and antibody first then lysates or antibody lysates and then beads? -  For how long the mixture was incubated? - Can we define the problem as no bands in IP or multiple bands; because loading 1000 ug protein per well in WB is way too high. If it is not true then I am presuming that this is the amount of protein (lysates) you have used to mix with antibody and beads. - 1/1000 dilution means the antibody was used at 1ug/ml which is very low; I would recommend using ab at 5 ug/ml and 10ug/ml. - Could you send us an image? I hope the suggestion of conc. will help to improve the results. I will also look forward receiving reply to my questions soon.

    Read More

    Abcam Scientific Support

    回复于 Oct 28 2011

    Question

    we currently using mouse IgG and Ran IgG for -ve control, however, the -ve staining comes faster and stronger than +ve control, we already dilute the IgG same concentration as those antibodies. but it still comes stronger. I would like to know the actual concentration for those antibodies to see whether there is an concentration unequally cause the strong -ve staining.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2012

    Answer

    AB18293; Rabbit anti- NRA52/LRH-1 Lot GR9515-6 = 0.290 mg/ml;  Lot: GR34910-1 = 0.220 mg/ml AB10685; Mouse anti- hnRNPA1 [4B10] Lot: GR14303-5 =  1 mg/ml AB6102; Mouse anti- hnRNPA2/B1 [DP3B3] Lot: GR21753-3 = 1 mg/ml AB16667 Rabbit anti- Ki67  Lot: GR40326-2 & Lot:GR17359-1. This product is supplied as a tissue culture supernatent. As there will be other proteins within this supernatent, specific antibody concentration can not be measured. Typically the concentration of tissue culture supernatants is between 10-50 ug/ml.

    Read More

    Abcam Scientific Support

    回复于 Dec 20 2012

    Question

    The order number is 15032.1.32-ET-MG.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 04 2011

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 979027 with ab31645. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Abcam Scientific Support

    回复于 Nov 04 2011

    Question

    I would prefer a different antibody than a vial from the same (since it is monoclonal, i'm not sure if the results would improve that much). Since the Anti-hnRNP A2B1 antibody (ab31645) works for ICC/IF it might work either with IP experiments. Being so, i would be appreciated if you could dispatch the one that i've refered. Thank you for your support,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2011

    Answer

    For dispatching the requested product, we would need the order number of original purchase. Could you please send this asap? Looking forward to hearing from you soon.  

    Read More

    Abcam Scientific Support

    回复于 Nov 02 2011

    Question

    What type of beads were used for IP? *Protein G-agarose beads* > How the IP was performed? Have you mixed antibody beads and lysates once, or beads and antibody first then lysates or antibody lysates and then beads? *Antibody (6ug) with 100ul of beads o.n., 4ºc in the next day, 1mg of protein was incubated during 1h at 4ºC* >For how long the mixture was incubated? *1h* >Can we define the problem as no bands in IP or multiple bands because loading 1000 ug protein per well in WB is way too high. If it is not true then I am presuming that this is the amount of protein (lysates) you have used to mix with antibody and beads. *1mg was used to the IP for the input, 50ug of protein* > 1/1000 dilution means the antibody was used at 1ug/ml which is very low I would recommend using ab at 5 ug/ml and 10ug/ml. *1/1000 was the dilution used in the 3 abreviews that were availabe together with the datasheet. So the dilution is not the problem at least in the input lane it was supposed to see a stronger band at 37kDa and none at 50kDa.* > Could you send us an image? *Sorry, i attached a file previously, but then the page have reloaded because i've spent more than 20min filling the form. How can i send you the image? I've tried to reply by e-mail but it was not delivered... Here's a link with the image that i wanted to attach: http://imageshack.us/f/31/hnrnpa2.png/

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2011

    Answer

    Thank you very much for sending the image and all the details. I acknowledge the time you have spent trying this antibody and convinced that the observed band size is not at 37 kDa. The antibody should be detecting the band at right size; the rat protein is approx 99% similar to human protein. I unfortunately can‘t compare the results with our own lab data because ab6102 was never tested with rat lysates. It may be worth going through the literature and comparing the results with other scientist’s findings. In order to proceed further in this case I can either send you a free of charge replacement of ab6102 from different lot or can also dispatch a different antibody e.g. ab31645 or ab24409 or ab64800; although these antibodies are not tested in IP however I don’t see a reason why these antibodies can’t be used in IP. If you are interested in trying these let me know I will then send you a vial of ab.  I also want to point out the background in IgG IP which kind of show similar pattern as IP of A2; I am not sure why this is, the IgG IP should be clean; one reason could be the high protein conc. as compare to the antibody. I will look forward to receiving your reply soon. Please do not hesitate to contact me if you have any question.  

    Read More

    Abcam Scientific Support

    回复于 Oct 28 2011

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