Anti-hnRNP A2B1抗体(ab223237)
Key features and details
- Goat polyclonal to hnRNP A2B1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-hnRNP A2B1抗体
参阅全部 hnRNP A2B1 一抗 -
描述
山羊多克隆抗体to hnRNP A2B1 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Dog, Pig, Orangutan
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免疫原
Synthetic peptide corresponding to Human hnRNP A2B1 aa 100-200 (internal sequence) (Cysteine residue). NP_002128.1; NP_112533.1.
Database link: P22626 -
阳性对照
- WB: MCF7 cell lysate; NIH/3T3 nuclear lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab223237 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223237于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous. -
序列相似性
Contains 2 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 507564 Cow
- Entrez Gene: 3181 Human
- Entrez Gene: 100045191 Mouse
- Entrez Gene: 53379 Mouse
- Entrez Gene: 100174392 Orangutan
- Entrez Gene: 362361 Rat
- Omim: 600124 Human
- SwissProt: Q2HJ60 Cow
see all -
别名
- Heterogeneous nuclear ribonucleoprotein A2 antibody
- Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
- Heterogeneous nuclear ribonucleoprotein B1 antibody
see all
图片
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Western blot - Anti-hnRNP A2B1 antibody (ab223237)Lane 1 : Anti-hnRNP A2B1 antibody (ab223237) at 0.1 µg/ml
Lanes 2-3 : Anti-hnRNP A2B1 antibody (ab223237) at 0.01 µg/ml
Lane 1 : NIH3T3 cell lysate (in RIPA buffer)
Lane 2 : Nuclear NIH3T3 cell lysate (in RIPA buffer)
Lane 3 : NIH3T3 cell lysate (in RIPA buffer) with Peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 36 kDaDetected by chemiluminescence.
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Western blot - Anti-hnRNP A2B1 antibody (ab223237)Anti-hnRNP A2B1 antibody (ab223237) at 0.3 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDaPrimary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (0)
ab223237 尚未被引用在任何文献中。
