Anti-hnRNP A2B1抗体(ab183654)
Key features and details
- Rabbit polyclonal to hnRNP A2B1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-hnRNP A2B1抗体
参阅全部 hnRNP A2B1 一抗 -
描述
兔多克隆抗体to hnRNP A2B1 -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human hnRNP A2B1 aa 100-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P22626 -
阳性对照
- A549, 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8030 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 and PC-12 whole cell lysates. U373 xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183654于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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WB |
1/5000 - 1/20000. Predicted molecular weight: 37 kDa.
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说明 |
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IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/20000. Predicted molecular weight: 37 kDa. |
靶标
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功能
Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous. -
序列相似性
Contains 2 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 3181 Human
- Entrez Gene: 100045191 Mouse
- Entrez Gene: 53379 Mouse
- Entrez Gene: 362361 Rat
- Omim: 600124 Human
- SwissProt: P22626 Human
- SwissProt: O88569 Mouse
- SwissProt: A7VJC2 Rat
see all -
别名
- Heterogeneous nuclear ribonucleoprotein A2 antibody
- Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
- Heterogeneous nuclear ribonucleoprotein B1 antibody
see all
图片
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All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa -
Immunohistochemical analysis of paraffin-embedded U373 xenograft labeling hnRNP A2B1 with ab183654 at 1/500 dilution.
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Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 37 kDa12 % SDS-PAGE
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All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH-3T3 whole cell lysate
Lane 5 : BCL-1 whole cell lysate
Lane 6 : Raw264.7 cell lysate
Lane 7 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa12 % SDS-PAGE
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Western blot analysis using ab183654 at 1/200 dilution detecting hnRNP A2B1 in immunoprecipitation pellets from 4500 µg A549 whole cell lysate with ab183654 at 2 µg. Lane 2 shows Control with 2 μg of preimmune rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab183654 被引用在 2 文献中.
- Yu B et al. Extracellular vesicles engineering by silicates-activated endothelial progenitor cells for myocardial infarction treatment in male mice. Nat Commun 14:2094 (2023). PubMed: 37055411
- Jimenez L et al. Culture conditions greatly impact the levels of vesicular and extravesicular Ago2 and RNA in extracellular vesicle preparations. J Extracell Vesicles 12:e12366 (2023). PubMed: 37885043