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重组RabMAb

重组Anti-hnRNP A1 (citrulline R97)抗体[EPR20176] - BSA and Azide free (ab271981)

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Dot Blot - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
  • Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
  • Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20176] to hnRNP A1 (citrulline R97) - BSA and Azide free
  • Suitable for: IP, WB, Dot blot
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-hnRNP A1 (citrulline R97)抗体[EPR20176] - BSA and Azide free
    参阅全部 hnRNP A1 一抗

  • 描述

    兔单克隆抗体[EPR20176] to hnRNP A1 (citrulline R97) - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Based on sequence homology we predict that this antibody may react with hnRNP A1L2.

  • 经测试应用

    适用于: IP, WB, Dot blotmore details

  • 种属反应性

    与反应: Human, Recombinant fragment

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HEK-293T transfected with PADI2 and PADI4, respectively, treated with CaCl2 and Ionomycin, whole cell lysates. IP: HEK-293T transfected with PADI4, treated with CaCl2 and Ionomycin, whole cell lysate.

  • 常规说明

    ab271981 is the carrier-free version of ab208028.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271981于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
Dot blot
Use at an assay dependent concentration.
说明
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
Dot blot
Use at an assay dependent concentration.

靶标

  • 功能

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

  • 序列相似性

    Contains 2 RRM (RNA recognition motif) domains.

  • 翻译后修饰

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.

  • 细胞定位

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt

  • 数据库链接

    • 别名

      • HNRNPA 1 antibody
      • Helix destabilizing protein antibody
      • Helix-destabilizing protein antibody
      • Heterogeneous nuclear ribonucleoprotein A1 antibody
      • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
      • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
      • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
      • hnRNP A1 antibody
      • hnRNP core protein A1 antibody
      • HNRNPA1 antibody
      • HNRPA1 antibody
      • MGC102835 antibody
      • Nuclear ribonucleoprotein particle A1 protein antibody
      • ROA1_HUMAN antibody
      • Single strand DNA binding protein UP1 antibody
      • Single strand RNA binding protein antibody
      • Single-strand RNA-binding protein antibody
      see all

    图片

    • Dot Blot - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
      Dot Blot - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)

      Dot blot analysis of hnRNP A1 (citrulline R97) labeled with ab208028 at 1/1000 dilution.

      Lane 1: hnRNP A1 (citrulline R97) peptide.
      Lane 2:hnRNP A1 (citrulline R97) non-citrulline peptide.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

      Blocking and dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208028).
    • Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)
      Immunoprecipitation - Anti-hnRNP A1 antibody [EPR20176] - BSA and Azide free (ab271981)

      hnRNP A1 (citrulline R97) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate with ab208028 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208028 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate 10 µg (Input). 
      Lane 2: ab208028 IP in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate. 
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208028 in HEK-293T transfected with GFP tagged PADI4 and treated with 10 mM CaCl2 for 2 hours, then 10 µM Ionomycin for 2 hours whole cell lysate.

      Exposure time: 10 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208028).

    • Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)
      Anti-hnRNP A1 (citrulline R97) antibody [EPR20176] - BSA and Azide free (ab271981)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271981？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271981 尚未被引用在任何文献中。

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