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Anti-hnRNP A1抗体[9H10] (ab5832)

Reviews (5)Q&A (2)References (58)
Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)
  • Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)
  • Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832)
  • Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)

Key features and details

  • Mouse monoclonal [9H10] to hnRNP A1
  • Suitable for: Flow Cyt, IHC-P, ELISA, WB, IP, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-hnRNP A1抗体[9H10]
    参阅全部 hnRNP A1 一抗

  • 描述

    小鼠单克隆抗体[9H10] to hnRNP A1

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, IHC-P, ELISA, WB, IP, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Full length hnRNPA1 native protein (partially purified) from HeLa cells (Human).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified from tissue culture supernatant.

  • 克隆

    单克隆

  • 克隆编号

    9H10

  • 骨髓瘤

    Sp2/0

  • 同种型

    IgG2b

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5832于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB (3)
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
IP
Use at an assay dependent concentration. This antibody does not IP the hnRNP complex.
ICC/IF (2)
Use at an assay dependent concentration.

See Abreview (April 2, 2007).
说明
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
IP
Use at an assay dependent concentration. This antibody does not IP the hnRNP complex.
ICC/IF
Use at an assay dependent concentration.

See Abreview (April 2, 2007).

靶标

  • 功能

    Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.

  • 序列相似性

    Contains 2 RRM (RNA recognition motif) domains.

  • 翻译后修饰

    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.

  • 细胞定位

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • HNRNPA 1 antibody
    • Helix destabilizing protein antibody
    • Helix-destabilizing protein antibody
    • Heterogeneous nuclear ribonucleoprotein A1 antibody
    • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
    • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
    • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
    • hnRNP A1 antibody
    • hnRNP core protein A1 antibody
    • HNRNPA1 antibody
    • HNRPA1 antibody
    • MGC102835 antibody
    • Nuclear ribonucleoprotein particle A1 protein antibody
    • ROA1_HUMAN antibody
    • Single strand DNA binding protein UP1 antibody
    • Single strand RNA binding protein antibody
    • Single-strand RNA-binding protein antibody
    see all

图片

  • Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)
    Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)
    All lanes : Anti-hnRNP A1 antibody [9H10] (ab5832) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?
    Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A1 antibody [9H10] (ab5832)
    Ab5832 staining human lung. Staining is localized to the nucleus.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)
    Western blot - Anti-hnRNP A1 antibody [9H10] (ab5832)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-hnRNP A1 antibody [9H10] (ab5832) at 1/1000 dilution

    Lane 1 : Mouse NSC34 whole cell lysate with control siRNA
    Lane 2 : Mouse NSC34 whole cell lysate with hnRNP A1 siRNA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye® 700DX-conjugated Donkey anti-Mouse IgG at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 38 kDa


    Exposure time: 1 minute


    Knockdown for 72 hours.

    See Abreview

  • Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832)
    Flow Cytometry - Anti-hnRNP A1 antibody [9H10] (ab5832)
    Overlay histogram showing Jurkat cells stained with ab5832 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5832, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)
    Immunoprecipitation - Anti-hnRNP A1 antibody [9H10] (ab5832)
    hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP A1 (ab5832) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5832.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 37kDa: hnRNP A1; 42kDa:We are unsure as to the identity of this extra band.

文献 (58)

发表研究结果有使用 ab5832？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5832 被引用在 58 文献中.

  • Li Z  et al. M2 Macrophage-Derived Exosomal lncRNA MIR4435-2HG Promotes Progression of Infantile Hemangiomas by Targeting HNRNPA1. Int J Nanomedicine 18:5943-5960 (2023). PubMed: 37881607
  • Tsai PL  et al. Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins. Cell Rep 41:111675 (2022). PubMed: 36417855
  • Prophet SM  et al. p97/UBXD1 Generate Ubiquitylated Proteins That Are Sequestered into Nuclear Envelope Herniations in Torsin-Deficient Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35563018
  • Luo Y  et al. KRAS mutant-driven SUMOylation controls extracellular vesicle transmission to trigger lymphangiogenesis in pancreatic cancer. J Clin Invest 132:N/A (2022). PubMed: 35579947
  • Zhang C  et al. Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis. J Immunol Res 2022:1917585 (2022). PubMed: 35692504
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-hnRNP A1 antibody [9H10]

 Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample
Human Cell lysate - whole cell (Human Embryonic Kidney)
Specification
Human Embryonic Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 18 2014

Western blot abreview for Anti-hnRNP A1 antibody [9H10]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34)
Loading amount
10 µg
Specification
NSC34
Gel Running Conditions
Reduced Denaturing (4-12% nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 13 2010

Western blot abreview for Anti-hnRNP A1 antibody [9H10]

 Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (cell type: IDH4)
Loading amount
5 µg
Specification
cell type: IDH4
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Elizabeth Kovacs

Verified customer

提交于 Jun 06 2006

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