Anti-HNF-4-alpha (phospho S313)抗体(ab78356)
Key features and details
- Rabbit polyclonal to HNF-4-alpha (phospho S313)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-HNF-4-alpha (phospho S313)抗体
参阅全部 HNF-4-alpha 一抗 -
描述
兔多克隆抗体to HNF-4-alpha (phospho S313) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human HNF-4-alpha (phospho S313).
-
阳性对照
- Extracts from HUVEC cells. Human liver carcinoma tissue. LOVO cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78356于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
|
|
| IHC-P |
1/50 - 1/100.
|
|
| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/500 - 1/1000. |
靶标
-
功能
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. -
疾病相关
Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
序列相似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
翻译后修饰
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 3172 Human
- Entrez Gene: 15378 Mouse
- Entrez Gene: 25735 Rat
- Omim: 600281 Human
- SwissProt: P41235 Human
- SwissProt: P49698 Mouse
- SwissProt: P22449 Rat
- Unigene: 116462 Human
see all -
别名
- FLJ39654 antibody
- FRTS4 antibody
- Hepatic nuclear factor 4 alpha antibody
see all
图片
-
Western blot - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)All lanes : Anti-HNF-4-alpha (phospho S313) antibody (ab78356) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab78356 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)Immunofluorescence analysis of LOVO cells using ab78356 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab78356 被引用在 1 文献中.
- Ceci L et al. Knockout of the Tachykinin Receptor 1 in the Mdr2-/- (Abcb4-/-) Mouse Model of Primary Sclerosing Cholangitis Reduces Biliary Damage and Liver Fibrosis. Am J Pathol 190:2251-2266 (2020). PubMed: 32712019