重组Anti-HNF-4-alpha抗体[EPR3649] (ab133343)
![Western blot - Anti-HNF-4-alpha antibody [EPR3649] (ab133343)](https://www.abcam.cn/ps/products/133/ab133343/Images/ab133343-146895-anti-hnf-4-alpha-antibody-epr3649-western-blot.jpg "Western blot - Anti-HNF-4-alpha antibody [EPR3649] (ab133343)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3649] to HNF-4-alpha
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HNF-4-alpha抗体[EPR3649]
参阅全部 HNF-4-alpha 一抗 -
描述
兔单克隆抗体[EPR3649] to HNF-4-alpha -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human HNF-4-alpha aa 200-300 (internal sequence). The exact sequence is proprietary.
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阳性对照
- HepG2 and Human fetal liver lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3649 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133343于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). |
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.
靶标
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功能
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. -
疾病相关
Defects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
序列相似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
翻译后修饰
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3172 Human
- Omim: 600281 Human
- SwissProt: P41235 Human
- Unigene: 116462 Human
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别名
- FLJ39654 antibody
- FRTS4 antibody
- Hepatic nuclear factor 4 alpha antibody
see all
图片
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Western blot - Anti-HNF-4-alpha antibody [EPR3649] (ab133343)All lanes : Anti-HNF-4-alpha antibody [EPR3649] (ab133343) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
![Anti-HNF-4-alpha antibody [EPR3649] (ab133343)](https://www.abcam.cn/ps/products/133/ab133343/Images/ab133343-2-benefits-of-recombinant-antibodies.png)
Anti-HNF-4-alpha antibody [EPR3649] (ab133343)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab133343 尚未被引用在任何文献中。