重组Anti-HNF-1B抗体[EPR6334(2)] (ab128912)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6334(2)] to HNF-1B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-HNF-1B抗体[EPR6334(2)]
参阅全部 HNF-1B 一抗 -
描述
兔单克隆抗体[EPR6334(2)] to HNF-1B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human HNF-1B aa 500-600. The exact sequence is proprietary.
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阳性对照
- Human fetal kidney and Caco 2 lysates. ICC/IF: human, rat and mouse liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6334(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-HNF-1B antibody [EPR6334(2)] - BSA and Azide free (ab248217)
- PE Anti-HNF-1B antibody [EPR6334(2)] (ab318375)
- APC Anti-HNF-1B antibody [EPR6334(2)] (ab318478)
- Alexa Fluor® 488 Anti-HNF-1B antibody [EPR6334(2)] (ab318581)
- Alexa Fluor® 647 Anti-HNF-1B antibody [EPR6334(2)] (ab318684)
- Alexa Fluor® 594 Anti-HNF-1B antibody [EPR6334(2)] (ab318787)
- Alexa Fluor® 555 Anti-HNF-1B antibody [EPR6334(2)] (ab318887)
- Alexa Fluor® 750 Anti-HNF-1B antibody [EPR6334(2)] (ab321020)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128912于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. -
疾病相关
Defects in HNF1B are the cause of renal cysts and diabetes syndrome (RCAD) [MIM:137920]; also called maturity-onset diabetes of the young type 5 (MODY5) or familial hypoplastic glomerulocystic kidney disease (GCKD). RCAD is an autosomal dominant disorder comprising non-diabetic renal disease resulting from abnormal renal development, and diabetes, which in some cases occurs earlier than age 25 years and is thus consistent with a diagnosis of maturity-onset diabetes of the young (MODY5). The renal disease is highly variable and includes renal cysts, glomerular tufts, aberrant nephrogenesis, primitive tubules, irregular collecting systems, oligomeganephronia, enlarged renal pelves, abnormal calyces, small kidney, single kidney, horseshoe kidney, and hyperuricemic nephropathy.
Defects in HNF1B may be rare genetic risk factor contributing to the development of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in HNF1B may be a cause of susceptibility to prostate cancer hereditary type 11 (HPC11) [MIM:611955]. It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. -
序列相似性
Belongs to the HNF1 homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6928 Human
- Entrez Gene: 21410 Mouse
- Entrez Gene: 25640 Rat
- Omim: 189907 Human
- SwissProt: P35680 Human
- SwissProt: P27889 Mouse
- SwissProt: P23899 Rat
- Unigene: 191144 Human
see all -
别名
- FJHN antibody
- Hepatocyte nuclear factor 1 beta antibody
- Hepatocyte nuclear factor 1-beta antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (ab128912)
Immunohistochemistry analysis of paraffin-embedded sections of rat liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (ab128912)
Immunohistochemistry analysis of paraffin-embedded sections of human liver tissue staining HNF-1B wth ab128912 with 1/100 dilution (4.6 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [EPR6334(2)] (ab128912)
Immunohistochemistry analysis of paraffin-embedded sections of mouse liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
All lanes : Anti-HNF-1B antibody [EPR6334(2)] (ab128912) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab128912 被引用在 3 文献中.
- Zhu Y et al. LncRNA4474 inhibits renal fibrosis by regulating hepatocyte nuclear factor-1β through miR-615 modulation. Cell Cycle 21:1233-1248 (2022). PubMed: 35230916
- Chan SC et al. Hepatocyte nuclear factor 1β suppresses canonical Wnt signaling through transcriptional repression of lymphoid enhancer-binding factor 1. J Biol Chem 295:17560-17572 (2020). PubMed: 33453998
- Liu Q et al. Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960 (2018). PubMed: 30154523