AB236759

Anti-HNF-1B 抗体 [CL0374]

Name: HNF-1B抗体[CL0374] (ab236759)| Abcam中文官网
Brand: Abcam Limited
SKU: AB236759
Price: 4864 CNY
Availability: InStock

Anti-HNF-1B antibody [CL0374]

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(5 Publications)

Mouse Monoclonal HNF-1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNF1B aa 1-150.

查看别名

TCF2, HNF1B, Hepatocyte nuclear factor 1-beta, HNF-1-beta, HNF-1B, Homeoprotein LFB3, Transcription factor 2, Variant hepatic nuclear factor 1, TCF-2, vHNF1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling HNF-1B (green) using ab236759 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human tonsil tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis. Negative control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human renal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human colorectal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human colon tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Paraffin-embedded human kidney tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

All lanes:

Western blot - Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/mL

Lane 1:

Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

Lane 2:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

All lanes:

Western blot - Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/mL

Lane 1:

Negative control, vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

HNF-1B over-expression (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK-293T cells) cell lysate

Predicted band size: 61 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

CL0374

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human HNF1B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P35680

表位

Binds to an epitope located within the peptide sequence TPPILKELQA as determined by overlapping synthetic peptides.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HNF-1B also known as hepatocyte nuclear factor 1-beta is a transcription factor with a molecular mass of approximately 56 kDa. This protein regulates gene expression by binding to specific DNA sequences influencing the transcriptional activity of target genes. HNF-1B is highly expressed in tissues such as the kidney liver pancreas and lung. It plays a critical role in the development and function of these organs exerting control over the expression of genes involved in their respective physiological processes.

Biological function summary

HNF-1B functions are integral to organ development and tissue-specific gene expression. It is part of the HNF family of transcription factors and forms complexes with other members like HNF-1A. HNF-1B regulates genes that are essential for epithelial cell differentiation and organ morphogenesis. This regulation ensures proper functioning of multiple organs highlighting its significance in maintaining organ health and homeostasis.

Pathways

HNF-1B is deeply integrated into the Wnt signaling pathway and renal-specific pathways. It modulates the expression of key components within these pathways such as promoting renal tubule development and function. HNF-1B interacts with other proteins like TCF7L2 and β-catenin facilitating its role in Wnt signaling. These interactions guide precise cellular responses allowing HNF-1B to coordinate complex biological processes like organogenesis and cellular differentiation.

Mutations in the gene encoding HNF-1B contribute to renal cysts and diabetes syndrome (RCAD) and maturity-onset diabetes of the young type 5 (MODY5). These genetic variations disrupt HNF-1B function leading to impaired organ development and function. In RCAD HNF-1B abnormalities link closely to kidney malformations and diabetic symptoms interacting with genes like PAX2 involved in kidney formation highlighting the molecular complexity associated with these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor that binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed : 17924661, PubMed : 7900999). Binds to the FPC element in the cAMP regulatory unit of the PLAU gene (By similarity). Transcriptional activity is increased by coactivator PCBD1 (PubMed : 24204001).

See full target information HNF1B

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Lupus science & medicine 11: PubMed39608815

2024

HNF-1β alleviates podocyte injury in lupus nephritis by maintaining endoplasmic reticulum homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Mei Zou,Jie Yu,Yuan-Yuan Ruan,Ying Xie,Xiao-Min An,Pei-Lei Chen,Ying-Qin Luo,Ming-Jun Shi,Miao Liu,Li-Fen Xu,Jun Liu,Bing Guo,Fan Zhang

JCI insight 7: PubMed34874920

2022

Angiotensin-(1-7)/MasR axis promotes migration of monocytes/macrophages with a regulatory phenotype to perform phagocytosis and efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Zaidan,Luciana P Tavares,Michelle A Sugimoto,Kátia M Lima,Graziele L Negreiros-Lima,Lívia Cr Teixeira,Thais C Miranda,Bruno Vs Valiate,Allysson Cramer,Juliana Priscila Vago,Gabriel H Campolina-Silva,Jéssica Am Souza,Laís C Grossi,Vanessa Pinho,Maria Jose Campagnole-Santos,Robson As Santos,Mauro M Teixeira,Izabela Galvão,Lirlândia P Sousa

Oncology letters 23:9 PubMed34820008

2021

Role of Lamin A and emerin in maintaining nuclear morphology in different subtypes of ovarian epithelial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Watabe,Sayaka Kobayashi,Mizuho Hatori,Yoshimi Nishijima,Naoki Inoue,Hayato Ikota,Akira Iwase,Hideaki Yokoo,Masanao Saio

Cell stem cell 28:1105-1124.e19 PubMed33915078

2021

Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Breunig,Jessica Merkle,Martin Wagner,Michael K Melzer,Thomas F E Barth,Thomas Engleitner,Johannes Krumm,Sandra Wiedenmann,Christian M Cohrs,Lukas Perkhofer,Gaurav Jain,Jana Krüger,Patrick C Hermann,Maximilian Schmid,Tamara Madácsy,Árpád Varga,Joscha Griger,Ninel Azoitei,Martin Müller,Oliver Wessely,Pamela G Robey,Sandra Heller,Zahra Dantes,Maximilian Reichert,Cagatay Günes,Christian Bolenz,Florian Kuhn,József Maléth,Stephan Speier,Stefan Liebau,Bence Sipos,Bernhard Kuster,Thomas Seufferlein,Roland Rad,Matthias Meier,Meike Hohwieler,Alexander Kleger

Oncology letters 20:80 PubMed32863913

2020

miR-375-3p inhibits the progression of laryngeal squamous cell carcinoma by targeting hepatocyte nuclear factor-1β.

Applications

Unspecified application

Species

Unspecified reactive species

Kunpeng Chang,Zhenxing Wei,Hua Cao

View all publications

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Anti-HNF-1B 抗体 [CL0374]

Anti-HNF-1B antibody [CL0374]

Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (AB236759)

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

Western blot - Anti-HNF-1B antibody [CL0374] (AB236759)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

表位