重组Anti-HN1抗体[EPR7364] (ab126705)
![Western blot - Anti-HN1 antibody [EPR7364] (ab126705)](https://www.abcam.cn/ps/products/126/ab126705/Images/ab126705-140646-anti-hn1-antibody-epr7364-western-blot.jpg "Western blot - Anti-HN1 antibody [EPR7364] (ab126705)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7364] to HN1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-HN1抗体[EPR7364]
参阅全部 HN1 一抗 -
描述
兔单克隆抗体[EPR7364] to HN1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide within Human HN1 aa 100-200. The exact sequence is proprietary.
-
阳性对照
- HepG2, Human testis, HeLa, Human fetal muscle, and Human thymus lysates; HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7364 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126705于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa). |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
-
组织特异性
Expressed in testis, skeletal muscle, thymus, prostate, colon, peripheral blood cells, brain and placenta. -
序列相似性
Belongs to the HN1 family. -
翻译后修饰
Isoform 3 initiator methionine is either acetylated or removed. In the latter case, the new N-terminal amino acid is then N-acetylated. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 51155 Human
- Entrez Gene: 15374 Mouse
- Entrez Gene: 287828 Rat
- SwissProt: Q9UK76 Human
- SwissProt: P97825 Mouse
- SwissProt: Q6AXU6 Rat
- Unigene: 532803 Human
- Unigene: 1775 Mouse
see all -
别名
- Androgen regulated protein 2 antibody
- Androgen-regulated protein 2 antibody
- ARM2 antibody
see all
图片
-
Western blot - Anti-HN1 antibody [EPR7364] (ab126705)All lanes : Anti-HN1 antibody [EPR7364] (ab126705) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human testis lysate
Lane 4 : Human fetal muscle lysate
Lane 5 : Human thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
![Flow Cytometry (Intracellular) - Anti-HN1 antibody [EPR7364] (ab126705)](https://www.abcam.cn/ps/products/126/ab126705/Images/ab126705-140645-anti-hn1-antibody-epr7364-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-HN1 antibody [EPR7364] (ab126705)ab126705 at 1/10 dilution staining HN1 in permeabilized HeLa cells by intracellular flow cytometry (red) compared to a rabbit IgG negative control (green).
-
![Anti-HN1 antibody [EPR7364] (ab126705)](https://www.abcam.cn/ps/products/126/ab126705/Images/ab126705-3-benefits-of-recombinant-antibodies.png)
Anti-HN1 antibody [EPR7364] (ab126705)
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab126705 被引用在 6 文献中.
- Chen YY et al. Circular RNA LPAR3 targets JPT1 via microRNA-513b-5p to facilitate glycolytic activation but repress prostate cancer radiosensitivity. Acta Biochim Pol 70:153-162 (2023). PubMed: 36929708
- Wang P et al. Circ-PGPEP1 augments renal cell carcinoma proliferation, Warburg effect, and distant metastasis. Acta Biochim Pol 70:703-711 (2023). PubMed: 37722013
- Wang R et al. The HN1/HMGB1 axis promotes the proliferation and metastasis of hepatocellular carcinoma and attenuates the chemosensitivity to oxaliplatin. FEBS J 289:6400-6419 (2022). PubMed: 35596723
- Tan S et al. Hesperidin administration suppresses the proliferation of lung cancer cells by promoting apoptosis via targeting the miR-132/ZEB2 signalling pathway. Int J Mol Med 46:2069-2077 (2020). PubMed: 33125117
- Pu J et al. hsa_circ_0000092 promotes hepatocellular carcinoma progression through up-regulating HN1 expression by binding to microRNA-338-3p. J Cell Mol Med N/A:N/A (2020). PubMed: 32077624
- Jia Q et al. HNRNPA1-mediated 3' UTR length changes of HN1 contributes to cancer- and senescence-associated phenotypes. Aging (Albany NY) 11:4407-4437 (2019). PubMed: 31257225