Anti-HMGN3抗体(ab72233)
Key features and details
- Rabbit polyclonal to HMGN3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HMGN3抗体 -
描述
兔多克隆抗体to HMGN3 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide from a region between residue 60 and 99 of human HMGN3, NP_004233.1.
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阳性对照
- Whole cell lysate from Hela cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72233于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function. -
组织特异性
Expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Low expression in liver, spleen, placenta and ovaries. -
序列相似性
Belongs to the HMGN family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 100069315 Horse
- Entrez Gene: 9324 Human
- Omim: 604502 Human
- SwissProt: Q15651 Human
- Unigene: 77558 Human
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别名
- DKFZp686E20226 antibody
- FLJ42187 antibody
- High mobility group nucleosomal binding domain 3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN3 antibody (ab72233)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling HMGN3 with ab72233 at 1/1000 (0.2µg/ml). Detection: DAB.
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Western blot - Anti-HMGN3 antibody (ab72233)All lanes : Anti-HMGN3 antibody (ab72233) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 11 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 16 kDa, 51 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-HMGN3 antibody (ab72233)Immunoprecipitation/ Western Blot of HMGN3.
Lane 1: ab72233 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72233 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab72233 被引用在 2 文献中.
- Sorin S et al. HMGN3 represses transcription of epithelial regulators to promote migration of cholangiocarcinoma in a SNAI2-dependent manner. FASEB J 36:e22345 (2022). PubMed: 35635715
- Yoffe Y et al. Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells. Genes Dev 30:1991-2004 (2016). PubMed: 27664238