重组Anti-HMGCS1抗体[EPR29166-11] - BSA and Azide free (ab317627)
![Western blot - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-542032-anti-hmgcs1-antibody-epr29166-11-western-blot-dld-1-human-colorectal-adenocarcinoma-epithelial-cell-whole-cell-lysate-50-ug.jpg "Western blot - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR29166-11] to HMGCS1 - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-HMGCS1抗体[EPR29166-11] - BSA and Azide free
参阅全部 HMGCS1 一抗 -
描述
兔单克隆抗体[EPR29166-11] to HMGCS1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, WBmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: DLD-1, PC-3, C2C12, Human liver cancer, Mouse stomach, Mouse testis, Rat stomach, Human stomach and Mouse colon lysates. IHC-P: Human liver cancer, Mouse colon, Mouse liver, Rat colon and Rat liver. IP: DLD-1 cell.
-
常规说明
ab317627 is the carrier-free version of ab317626
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR29166-11 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317627于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
靶标
-
功能
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. -
通路
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3. -
序列相似性
Belongs to the HMG-CoA synthase family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 3157 Human
- Entrez Gene: 100040592 Mouse
- Entrez Gene: 208715 Mouse
- Entrez Gene: 29637 Rat
- Omim: 142940 Human
- SwissProt: Q01581 Human
- SwissProt: Q8JZK9 Mouse
- SwissProt: P17425 Rat
see all -
别名
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody
- 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
- cytoplasmic antibody
see all
图片
-
Western blot - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)All lanes : Anti-HMGCS1 antibody [EPR29166-11] (ab317626) at 1/1000 dilution
Lane 1 : DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : C2C12 (mouse myoblast) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 180 secondsThis data was developed using ab317626, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: LNCaP (PMID: 29163687), PC-3 (PMID: 29163687).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Western blot - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-542033-anti-hmgcs1-antibody-epr29166-11-western-blot-human-liver-tissue-lysate-50-ug.jpg)
Western blot - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)All lanes : Anti-HMGCS1 antibody [EPR29166-11] (ab317626) at 1/1000 dilution
Lane 1 : Human liver tissue lysate at 50 µg
Lane 2 : Human liver cancer tissue lysate at 50 µg
Lane 3 : Human skeletal muscle tissue lysate at 50 µg
Lane 4 : Mouse stomach tissue lysate at 50 µg
Lane 5 : Mouse testis tissue lysate at 50 µg
Lane 6 : Mouse skeletal muscle tissue lysate at 50 µg
Lane 7 : Rat stomach tissue lysate at 50 µg
Lane 8 : Rat skeletal muscle tissue lysate at 50 µg
Lane 9 : Human stomach tissue lysate at 20 µg
Lane 10 : Mouse colon tissue lysate at 50 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDaThis data was developed using ab317626, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: skeletal muscle, human liver (PMID: 36356901).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-3: 3 seconds, Lane 4-10: 180 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-2-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-human-liver-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling HMGCS1 with ab317626 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human liver cancer (PMID: 36356901).
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-3-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-mouse-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling HMGCS1 with ab317626 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse colon.
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-4-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HMGCS1 with ab317626 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse liver.
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-5-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling HMGCS1 with ab317626 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat colon.
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-6-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-rat-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling HMGCS1 with ab317626 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in rat liver.
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-7-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HMGCS1 with ab317626 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining in human liver (PMID: 36356901).
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-8-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-mouse-skeletal-muscl.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling HMGCS1 with ab317626 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining in mouse skeletal muscle.
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-9-anti-hmgcs1-antibody-epr29166-11-immunohistochemistry-rat-skeletal-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling HMGCS1 with ab317626 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining in rat skeletal muscle.
The section was incubated with ab317626 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunoprecipitation - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-10-anti-hmgcs1-antibody-epr29166-11-immunoprecipitation-dld-1.jpg)
Immunoprecipitation - Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)This data was developed using ab317626, the same antibody clone in a different buffer formulation.
HMGCS1 was immunoprecipitated from 0.35 mg DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab317626 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317626 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab317626 IP in DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317626 in DLD-1 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
-
![Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)](https://www.abcam.cn/ps/products/317/ab317627/Images/ab317627-1-BenefitsofRecombinantantibodies.jpg)
Anti-HMGCS1 antibody [EPR29166-11] - BSA and Azide free (ab317627)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (0)
ab317627 尚未被引用在任何文献中。
