重组Anti-HMGB2抗体[EPR6301] (ab124670)
![Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)](https://www.abcam.cn/ps/products/124/ab124670/Images/ab124670-448902-anti-hmgb2-antibody-epr6301-western-blot-hek-293t-lysates.jpg "Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6301] to HMGB2
- Suitable for: ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-HMGB2抗体[EPR6301]
参阅全部 HMGB2 一抗 -
描述
兔单克隆抗体[EPR6301] to HMGB2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human HMGB2 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: HEK-293T, HAP1, K562, HeLa and PC12 cell lysates. ICC/IF: PC-12 cells. IHC-P: Human breast tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 6.03 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6301 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124670于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500.
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| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
1/500. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 24 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
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功能
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). -
序列相似性
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3148 Human
- Entrez Gene: 97165 Mouse
- Entrez Gene: 29395 Rat
- Entrez Gene: 498072 Rat
- Omim: 163906 Human
- SwissProt: P26583 Human
- SwissProt: P30681 Mouse
- SwissProt: P52925 Rat
see all -
别名
- C80539 antibody
- High mobility group (nonhistone chromosomal) protein 2 antibody
- High mobility group box 2 antibody
see all
图片
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Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/2000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : HMGB2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDaLanes 1- 2: Merged signal (red and green). Green - ab124670 observed at 24 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab124670 was shown to react with HMGB2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266358 (knockout cell lysate ab257156) was used. Wild-type HEK-293T and HMGB2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB2 antibody [EPR6301] (ab124670)](https://www.abcam.cn/ps/products/124/ab124670/Images/ab124670-138401-anti-hmgb2-antibody-epr6301-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB2 antibody [EPR6301] (ab124670)ab124670, at a 1/250 dilution, staining HMGB2 in paraffin embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-HMGB2 antibody [EPR6301] (ab124670)](https://www.abcam.cn/ps/products/124/ab124670/Images/ab124670-261201-anti-hmgb2-antibody-epr6301-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HMGB2 antibody [EPR6301] (ab124670)Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling HMGB2 with ab124670 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
![Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)](https://www.abcam.cn/ps/products/124/ab124670/Images/ab124670-312271-anti-hmgb2-antibody-epr6301-western-blot.jpg)
Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HMGB2 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 24 kDaLanes 1 - 4: Merged signal (red and green). Green - ab124670 observed at 24 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab124670 was shown to specifically react with HMGB2 in wild-type HAP1 cells as signal was lost in HMGB2 knockout cells. Wild-type and HMGB2 knockout samples were subjected to SDS-PAGE. Ab124670 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)](https://www.abcam.cn/ps/products/124/ab124670/Images/ab124670-138402-anti-hmgb2-antibody-epr6301-western-blot.jpg)
Western blot - Anti-HMGB2 antibody [EPR6301] (ab124670)All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution
Lane 1 : K562 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : PC12 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa -
![OI-RD Scanning - Anti-HMGB2 antibody [EPR6301] (ab124670)](https://www.abcam.cn/ps/products/124/ab124670/Images/ab124670-138400-anti-hmgb2-antibody-epr6301.jpg)
OI-RD Scanning - Anti-HMGB2 antibody [EPR6301] (ab124670)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-HMGB2 antibody [EPR6301] (ab124670)](https://www.abcam.cn/ps/products/124/ab124670/Images/ab124670-7-benefits-of-recombinant-antibodies.png)
Anti-HMGB2 antibody [EPR6301] (ab124670)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab124670 被引用在 17 文献中.
- Chen P et al. Single-Cell and Spatial Transcriptomics Decodes Wharton's Jelly-Derived Mesenchymal Stem Cells Heterogeneity and a Subpopulation with Wound Repair Signatures. Adv Sci (Weinh) 10:e2204786 (2023). PubMed: 36504438
- Fan C et al. Puerarin from Pueraria lobate attenuates ischemia-induced cardiac injuries and inflammation in vitro and in vivo: The key role of miR-130a-5p/HMGB2 pathway. Chem Biol Drug Des 101:952-961 (2023). PubMed: 36601671
- Ma W et al. Circular RNA circ_UBAP2 facilitates the progression of osteosarcoma by regulating microRNA miR-637/high-mobility group box (HMGB) 2 axis. Bioengineered 13:4411-4427 (2022). PubMed: 35114890
- Yano K et al. Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration. Sci Rep 12:11962 (2022). PubMed: 35831365
- Qiu X et al. Identification of HMGB2 associated with proliferation, invasion and prognosis in lung adenocarcinoma via weighted gene co-expression network analysis. BMC Pulm Med 22:310 (2022). PubMed: 35962344