Anti-HMGA2抗体(ab97276)
Key features and details
- Rabbit polyclonal to HMGA2
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HMGA2抗体
参阅全部 HMGA2 一抗 -
描述
兔多克隆抗体to HMGA2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human HMGA2 aa 1-93 (N terminal).
Database link: P52926 -
阳性对照
- WB: MOLT-4, HepG2, HCT116, HeLa, A431, NIH/3T3, Raw264.7, C2C12, PC-12, Rat2 whole cell lysate. IP: HepG2 cells. ICC/IF: MCF7, HeLa cells. IHC-P: Rat brain, rat duodenum, human HuCCT1 xenograft, C2C12 xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97276于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| WB | (2) |
1/500 - 1/50000. Predicted molecular weight: 12 kDa.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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IHC-P
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
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WB
1/500 - 1/50000. Predicted molecular weight: 12 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. -
疾病相关
Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma. -
序列相似性
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
发展阶段
Expressed predominantly during embryogenesis. -
翻译后修饰
Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8091 Human
- Entrez Gene: 15364 Mouse
- Entrez Gene: 84017 Rat
- Omim: 600698 Human
- SwissProt: P52926 Human
- SwissProt: P52927 Mouse
- Unigene: 505924 Human
- Unigene: 157190 Mouse
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别名
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
see all
图片
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : A431 whole cell extract
Lane 2 : HeLa whole cell extract
Lane 3 : HepG2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded C2C12 xenograft tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)HeLa cells stained for HMGA2 (green) using ab97276 at 1/500 dilution in ICC/IF.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1 : NIH/3T3 whole cell extract
Lane 2 : Raw264.7 whole cell extract
Lane 3 : C2C12 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded human HuCCT1 xenograft tissue stained for HMGA2 using ab97276 at 1/250 dilution in immunohistochemical analysis.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1 : PC-12 whole cell extract
Lane 2 : Rat2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded rat duodenum tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/10000 dilution + HCT116 whole cell extract at 30 µg
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded rat brain tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-HMGA2 antibody (ab97276)Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody. Western blot analysis was performed using HMGA2 antibody at 1/500 dilution. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Input
Lane 2: Immunoprecipitation using control IgG
Lane 3: Immunoprecipitation using 5 μg ab97276
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 12 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : HepG2
Lane 2 : HCT116
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa -
Western blot - Anti-HMGA2 antibody (ab97276)This image is courtesy of an anonymous abreview.Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + HeLa whole cell lysate at 25 µg
Secondary
Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution
Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab97276 被引用在 45 文献中.
- Li DK et al. Exosomal HMGA2 protein from EBV-positive NPC cells destroys vascular endothelial barriers and induces endothelial-to-mesenchymal transition to promote metastasis. Cancer Gene Ther 29:1439-1451 (2022). PubMed: 35388172
- Dong Q et al. Circular ribonucleic acid nucleoporin 98 knockdown alleviates high glucose-induced proliferation, fibrosis, inflammation and oxidative stress in human glomerular mesangial cells by regulating the microribonucleic acid-151-3p-high mobility group AT-hook 2 axis. J Diabetes Investig 13:1303-1315 (2022). PubMed: 35482475
- Hong Y et al. High-frequency repetitive transcranial magnetic stimulation (rTMS) protects against ischemic stroke by inhibiting M1 microglia polarization through let-7b-5p/HMGA2/NF-κB signaling pathway. BMC Neurosci 23:49 (2022). PubMed: 35927640
- Liang C et al. P300-dependent acetylation of histone H3 is required for epidermal growth factor receptor-mediated high-mobility group protein A2 transcription in hepatocellular carcinoma. Cancer Sci 112:679-690 (2021). PubMed: 33164305
- Liu K et al. Circular RNA 100146 Promotes Colorectal Cancer Progression by the MicroRNA 149/HMGA2 Axis. Mol Cell Biol 41:N/A (2021). PubMed: 33257506