Anti-HMGA2 抗体

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody (AB97276)

15% SDS-PAGE

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/1000 dilution

Lane 1:

NIH/3T3 whole cell extract at 30 µg

Lane 2:

Raw264.7 whole cell extract at 30 µg

Lane 3:

C2C12 whole cell extract at 30 µg

Predicted band size: 12 kDa

false

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin-embedded rat duodenum and rat brain tissue stained for HMGA2 - ChIP grade protein with ab97276 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin-embedded H1299 xenograft tissue stained for HMGA2 with ab97276 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (AB97276)

ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight^® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin embedded human HuCCT1 xenograft tissue stained for HMGA2 using ab97276 at 1/250 dilution in immunohistochemical analysis.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (AB97276)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling HMGA2 with ab320849 at 1/5000 (0.10 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing nuclear staining in HepG2 cell line
(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : HT-29 (PMID : 26964871).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Left Panel is applied with Anti-HMGA2 antibody [RM1215] (ab320849) at 1/5000 dilution (0.10 ug/ml);
Right panel is applied with Anti-HMGA2 antibody (ab97276) at 1/1000 dilution (0.08 ug/ml).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (AB97276)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins HeLa cells labelling HMGA2 protein at nucleus using ab97276 at a 1/500 dilution (Green). Alpha tubulin, a cytoskeleton marker stained by anti-alpha tubulin antibody at a 1/1000 dilution (Red).

• IP

Supplier Data

Immunoprecipitation - Anti-HMGA2 antibody (AB97276)

Paraffin-embedded mouse duodenum and mouse brain tissue stained for HMGA2 - ChIP grade protein with ab97276 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

All lanes:

Immunoprecipitation - Anti-Integrin beta 1/CD29 antibody [HL1255] (<a href='/products/primary-antibodies/integrin-beta-1-cd29-antibody-hl1255-ab308384'>ab308384</a>)

All lanes:

A431 whole cell extracts

Secondary

All lanes:

HRP-conjugated anti rabbit IgG antibody

Predicted band size: 88 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin embedded rat brain tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin-embedded mouse duodenum and mouse brain tissue stained for HMGA2 - ChIP grade protein with ab97276 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin-embedded rat duodenum and rat brain tissue stained for HMGA2 - ChIP grade protein with ab97276 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin embedded rat duodenum tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (AB97276)

Paraffin embedded C2C12 xenograft tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody (AB97276)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

All lanes:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 12 kDa

true

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody (AB97276)

15% SDS-PAGE

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

All lanes:

HCT116 whole cell extract at 30 µg

Predicted band size: 12 kDa

false

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody (AB97276)

15% SDS-PAGE

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

Lane 1:

A431 whole cell extract at 30 µg

Lane 2:

HeLa whole cell extract at 30 µg

Lane 3:

HepG2 whole cell extract at 30 µg

Predicted band size: 12 kDa

false

• WB

AbReview28009****

Western blot - Anti-HMGA2 antibody (AB97276)

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution

Predicted band size: 12 kDa

Observed band size: 18 kDa

false

Exposure time: 10min

This image is courtesy of an anonymous abreview.

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody (AB97276)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/50000 dilution

Lane 1:

Non-transfected (-) HeLa whole cell extracts at 30 µg

Lane 2:

Transfected (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 12 kDa

false

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody (AB97276)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

All lanes:

Rat2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 12 kDa

false

• WB

Supplier Data

Western blot - Anti-HMGA2 antibody (AB97276)

15% SDS-PAGE

All lanes:

Western blot - Anti-HMGA2 antibody (ab97276) at 1/1000 dilution

Lane 1:

PC-12 whole cell extract at 30 µg

Lane 2:

Rat2 whole cell extract at 30 µg

Predicted band size: 12 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-HMGA2 antibody (AB97276)

Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody. Western blot analysis was performed using HMGA2 antibody at 1/500 dilution. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Input

Lane 2 : Immunoprecipitation using control IgG

Lane 3 : Immunoprecipitation using 5 μg ab97276

All lanes:

Immunoprecipitation - Anti-HMGA2 antibody (ab97276)

Predicted band size: 12 kDa

false