重组Anti-HMG4抗体[EPR2838] (ab133258)
![Western blot - Anti-HMG4 antibody [EPR2838] (ab133258)](https://www.abcam.cn/ps/products/133/ab133258/Images/HMG4-Primary-antibodies-ab133258-1.JPG "Western blot - Anti-HMG4 antibody [EPR2838] (ab133258)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2838] to HMG4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HMG4抗体[EPR2838]
参阅全部 HMG4 一抗 -
描述
兔单克隆抗体[EPR2838] to HMG4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human HMG4 aa 50-150. The exact sequence is proprietary.
Database link: O15347 -
阳性对照
- HeLa cell lysate, 293T cell lysate, Human placenta tissue lysate, JAR cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2838 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Binds preferentially single-stranded DNA and unwinds double stranded DNA. -
组织特异性
Expressed predominantly in placenta. -
序列相似性
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3149 Human
- Entrez Gene: 15354 Mouse
- Entrez Gene: 305373 Rat
- Omim: 300193 Human
- SwissProt: O15347 Human
- SwissProt: O54879 Mouse
- Unigene: 19114 Human
- Unigene: 336087 Mouse
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别名
- chromosomal protein, Nonhistone, HMG4 antibody
- High mobility group (nonhistone chromosomal) protein 4 antibody
- High mobility group box 3 antibody
see all
图片
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Western blot - Anti-HMG4 antibody [EPR2838] (ab133258)All lanes : Anti-HMG4 antibody [EPR2838] (ab133258) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human placenta lysate
Lane 4 : JAR cell lysate
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution
Predicted band size: 23 kDa -
![Anti-HMG4 antibody [EPR2838] (ab133258)](https://www.abcam.cn/ps/products/133/ab133258/Images/ab133258-2-benefits-of-recombinant-antibodies.png)
Anti-HMG4 antibody [EPR2838] (ab133258)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133258 被引用在 1 文献中.
- Yu H et al. LINC00504 Promotes the Malignant Biological Behavior of Breast Cancer Cells by Upregulating HMGB3 via Decoying MicroRNA-876-3p. Cancer Manag Res 13:1803-1815 (2021). PubMed: 33654429