重组Anti-HMG4抗体[EP2839Y] (ab75782)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2839Y] to HMG4
- Suitable for: IP, ICC/IF, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-HMG4抗体[EP2839Y]
参阅全部 HMG4 一抗 -
描述
兔单克隆抗体[EP2839Y] to HMG4 -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human bladder, Human placenta, Mouse brain, Mouse bladder, Rat brain, Rat bladder, Rat placenta and HeLa lysates. IHC-P: Human placenta, Mouse liver, and Rat liver tissues. ICC/IF: 293T cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2839Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75782于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/20.
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ICC/IF |
1/250.
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Flow Cyt (Intra) |
1/20.
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|
WB |
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
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|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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IP
1/20. |
ICC/IF
1/250. |
Flow Cyt (Intra)
1/20. |
WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Binds preferentially single-stranded DNA and unwinds double stranded DNA. -
组织特异性
Expressed predominantly in placenta. -
序列相似性
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3149 Human
- Entrez Gene: 15354 Mouse
- Entrez Gene: 305373 Rat
- Omim: 300193 Human
- SwissProt: O15347 Human
- SwissProt: O54879 Mouse
- Unigene: 19114 Human
- Unigene: 336087 Mouse
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别名
- chromosomal protein, Nonhistone, HMG4 antibody
- High mobility group (nonhistone chromosomal) protein 4 antibody
- High mobility group box 3 antibody
see all
图片
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All lanes : Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/1000 dilution (Purified)
Lane 1 : Human bladder lysate
Lane 2 : Human placenta lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse bladder lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat bladder lysate
Lane 7 : Rat placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling HMG4 with Purified ab75782 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling HMG4 with Purified ab75782 at 1/250 dilution (0.452 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HMG4 with Purified ab75782 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Purified ab75782 at 1/20 dilution (0.5µg) immunoprecipitating HMG4 in HeLa whole cell lysate.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2: ab75782 + HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75782 in HeLa whole cell lysate.VeriBlot for IP Detection Reagent (HRP)(ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.Observed band size: 25 kDa
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Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling HMG4 with Purified ab75782 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling HMG4 with Purified ab75782 at 1/100 dilution (1.13 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab75782 被引用在 7 文献中.
- Ma H et al. HMGB3 promotes PARP inhibitor resistance through interacting with PARP1 in ovarian cancer. Cell Death Dis 13:263 (2022). PubMed: 35332131
- Zhang K et al. Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis. Cell Death Dis 12:554 (2021). PubMed: 34050127
- Sun J et al. Down-regulation of SNHG16 alleviates the acute lung injury in sepsis rats through miR-128-3p/HMGB3 axis. BMC Pulm Med 21:191 (2021). PubMed: 34092219
- Saghafinia S et al. Cancer Cells Retrace a Stepwise Differentiation Program during Malignant Progression. Cancer Discov 11:2638-2657 (2021). PubMed: 33910926
- Wu K et al. Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinoma. Aging (Albany NY) 13:14304-14321 (2021). PubMed: 34016791
- Yuan W et al. Silencing of Long Non-Coding RNA X Inactive Specific Transcript (Xist) Contributes to Suppression of Bronchopulmonary Dysplasia Induced by Hyperoxia in Newborn Mice via microRNA-101-3p and the transforming growth factor-beta 1 (TGF-ß1)/Smad3 Axis. Med Sci Monit 26:e922424 (2020). PubMed: 33070148
- Wang LK et al. Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein. Technol Cancer Res Treat 17:1533033818806475 (2018). PubMed: 30343649