AB17984

Anti-HLTF 抗体

Name: HLTF抗体 (ab17984)| Abcam中文官网
Brand: Abcam Limited
SKU: AB17984
Price: 4734 CNY
Availability: InStock
Rating: 3 (2 reviews)

Anti-HLTF antibody

(2 Reviews)

(17 Publications)

Rabbit Polyclonal HLTF antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human HLTF aa 600-700.

查看别名

HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1, HLTF, Helicase-like transcription factor, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3

2 Images

Unknown

Western blot - Anti-HLTF antibody (AB17984)

Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1 : 20 μg of HeLa nuclear extract
Lane 2 : 50 μg of HeLa nuclear extract
LAne 3 : 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 μg/mg of lysate.
Anti-SMARCA3 (ab17984) was used for western blot analysis at 1/5,000.
Detection : chemiluminescence with an exposure time of 4 minutes.

All lanes:

Western blot - Anti-HLTF antibody (ab17984)

Predicted band size: 113 kDa

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Unknown

Western blot - Anti-HLTF antibody (AB17984)

All lanes:

Western blot - Anti-HLTF antibody (ab17984) at 0.5 µg/mL

Lane 1:

NIH 3T3 whole cell lysate at 50 µg

Lane 2:

TCMK-1 whole cell lysate at 50 µg

Lane 3:

4T1 whole cell lysate at 50 µg

Lane 4:

CT26.WT whole cell lysate at 50 µg

Predicted band size: 113 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

Synthetic Peptide within Human HLTF aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q14527

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.

See full target information HLTF

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5789 PubMed38987539

2024

HLTF disrupts Cas9-DNA post-cleavage complexes to allow DNA break processing.

Applications

Unspecified application

Species

Unspecified reactive species

Giordano Reginato,Maria Rosaria Dello Stritto,Yanbo Wang,Jingzhou Hao,Raphael Pavani,Michael Schmitz,Swagata Halder,Vincent Morin,Elda Cannavo,Ilaria Ceppi,Stefan Braunshier,Ananya Acharya,Virginie Ropars,Jean-Baptiste Charbonnier,Martin Jinek,Andrè Nussenzweig,Taekjip Ha,Petr Cejka

Science (New York, N.Y.) 385:eado3867 PubMed38900911

2024

Structure and repair of replication-coupled DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Raphael Pavani,Veenu Tripathi,Kyle B Vrtis,Dali Zong,Raj Chari,Elsa Callen,Ajith V Pankajam,Gang Zhen,Gabriel Matos-Rodrigues,Jiajie Yang,Shuheng Wu,Giordano Reginato,Wei Wu,Petr Cejka,Johannes C Walter,André Nussenzweig

Biology direct 19:2 PubMed38163902

2024

DTX2 promotes glioma development via regulation of HLTF.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Li,Yang Chen,Biao Yang,Ziao Li,Peize Li,Yu Chen,Jiayu Li,Jianhang He,Yongqiang Wu,Yanqi Sun,Xiaogang Wang,Xiaolong Guo,Wenju Zhang,Yuanli Zhao,Geng Guo

Nucleic acids research 51:12207-12223 PubMed37897354

2023

Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqin Zhao,Kaiping Hou,Youhang Li,Shuailin Hao,Yu Liu,Yinan Na,Chao Li,Jian Cui,Xingzhi Xu,Xiaohua Wu,Hailong Wang

Life science alliance 5: PubMed35905994

2022

RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Rie Kanao,Hidehiko Kawai,Toshiyasu Taniguchi,Minoru Takata,Chikahide Masutani

Nature communications 13:66 PubMed35013187

2022

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Roy Matkovic,Marina Morel,Sophie Lanciano,Pauline Larrous,Benjamin Martin,Fabienne Bejjani,Virginie Vauthier,Maike M K Hansen,Stéphane Emiliani,Gael Cristofari,Sarah Gallois-Montbrun,Florence Margottin-Goguet

PloS one 16:e0251132 PubMed34010296

2021

Helicase-like transcription factor-deletion from the tumor microenvironment in a cell line-derived xenograft model of colorectal cancer reprogrammed the human transcriptome-S-nitroso-proteome to promote inflammation and redirect metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca A Helmer,Raul Martinez-Zaguilan,Gurvinder Kaur,Lisa A Smith,Jannette M Dufour,Beverly S Chilton

Molecular cell 80:327-344.e8 PubMed32966758

2020

PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Jin Kim,Seo Yun Lee,Ji-Hye Choi,Hyun Goo Woo,Blerta Xhemalce,Kyle M Miller

Nucleic acids research 46:11340-11356 PubMed30335157

2018

Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Masuda,Satoshi Mitsuyuki,Rie Kanao,Asami Hishiki,Hiroshi Hashimoto,Chikahide Masutani

Cell host & microbe 24:447-460.e11 PubMed30122656

2018

High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Katie Nightingale,Kai-Min Lin,Benjamin J Ravenhill,Colin Davies,Luis Nobre,Ceri A Fielding,Eva Ruckova,Alice Fletcher-Etherington,Lior Soday,Hester Nichols,Daniel Sugrue,Eddie C Y Wang,Pablo Moreno,Yagnesh Umrania,Edward L Huttlin,Robin Antrobus,Andrew J Davison,Gavin W G Wilkinson,Richard J Stanton,Peter Tomasec,Michael P Weekes

View all publications

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Anti-HLTF 抗体

Anti-HLTF antibody

Western blot - Anti-HLTF antibody (AB17984)

Western blot - Anti-HLTF antibody (AB17984)